Antibodies : a practical approach

著者

書誌事項

Antibodies : a practical approach

edited by D. Catty

（The practical approach series）

IRL Press, 1988-1989

  • v. 1
  • v. 1 : soft
  • v. 2
  • v. 2 : soft

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注記

Includes bibliographies and index

内容説明・目次

巻冊次

v. 2 ISBN 9780199630189

内容説明

This book is the second of a two-volume series covering the production of antibodies and their major applications. Volume I concentrates on how to raise monoclonal and polyclonal antibodies and how to use them in a set of universally applied tests. Now Volume II extends this repertoire of antibody applications with detailed accounts of important labelled antibody techniques using radioisotopes, fluorochromes and enzymes, along with descriptions of red cell and tissue typing. Specific techniques described here include assays with radiolabels, ELISA and related enzyme immunoassays, immunoperoxidase methods, immunofluorescence, fluorescence-activated cell sorting, tumor immunodetection, blood group serology, and HLA serology. The volume will be invaluable for any researcher interested in making greater use of antibodies as research tools, or in updating their present methodology. The detail given is sufficient to enable immediate use of the protocols.

目次

  • G.D. Poole: Antibodies in blood group serology
  • T.C. Ray: Antibodies in HLA serology
  • D. Catty & G. Murphy: Immunoassays using radiolabels
  • D. Catty & C. Raykundalia: ELISA and related enzyme immunoassays
  • E.L. Jones & J. Gregory: Immunoperoxidase methods
  • Immunofluorescence
  • A. Vaughan & A. Milner: Fluorescence activated cell sorting
  • G.D. Thomas: Antibodies for tumour immunodetection and method for antibody radiolabelling
巻冊次

v. 2 : soft ISBN 9780199630196

内容説明

This book is the second of a two-volume series covering the production of antibodies and their major applications. Volume I concentrates on how to raise monoclonal and polyclonal antibodies and how to use them in a set of universally applied tests. Now Volume II extends this repertoire of antibody applications with detailed accounts of important labelled antibody techniques using radioisotopes, fluorochromes and enzymes, along with descriptions of red cell and tissue typing. Specific techniques described here include assays with radiolabels, ELISA and related enzyme immunoassays, immunoperoxidase methods, immunofluorescence, fluorescence-activated cell sorting, tumor immunodetection, blood group serology, and HLA serology. The volume will be invaluable for any researcher interested in making greater use of antibodies as research tools, or in updating their present methodology. The detail given is sufficient to enable immediate use of the protocols.

目次

  • G.D. Poole: Antibodies in blood group serology
  • T.C. Ray: Antibodies in HLA serology
  • D. Catty & G. Murphy: Immunoassays using radiolabels
  • D. Catty & C. Raykundalia: ELISA and related enzyme immunoassays
  • E.L. Jones & J. Gregory: Immunoperoxidase methods
  • Immunofluorescence
  • A. Vaughan & A. Milner: Fluorescence activated cell sorting
  • G.D. Thomas: Antibodies for tumour immunodetection and method for antibody radiolabelling
巻冊次

v. 1 : soft ISBN 9780947946852

内容説明

Antibodies have become the preferred, and often essential, tools to identify, quantify and probe the structure and biological properties of an enormous range of antigenic molecules. This volume describes the properties of antibodies and antigens and provides detailed protocols for the production and quality control of polyclonal, murine monoclonal and human monoclonal antibodies. Step-by-step descriptions are given of antibody methods. In addition to giving precise methodological details, the contributors consider the applications and appropriate use of these techniques.

目次

  • Properties of antibodies and antigens
  • Production and quality control of polyclonal antibodies
  • Murine monoclonal antibodies
  • Human monoclonal antibodies
  • Immunoaffinity chromatography
  • Gel immunodiffusion, immunoeletrophoresis and immunostaining methods
  • Haemagglutination and haemolysis assays.
巻冊次

v. 1 ISBN 9780947946869

内容説明

This book is the first part of a two-volume series covering production of antibodies and their major applications.

目次

  • Properties of antibodies and antigens
  • Production and quality control of polyclonal antibodies
  • Murine monoclonal antibodies
  • Human monoclonal antibodies
  • Imunoaffinity chromatography
  • Gel immunodiffusion, immunoeletrophoresis and immunostaining methods
  • Haemagglutination and haemolysis assays

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