Fluorescent analogs, labeling cells, and basic microscopy
著者
書誌事項
Fluorescent analogs, labeling cells, and basic microscopy
(Methods in cell biology / edited by David M. Prescott, v. 29 . Fluorescence microscopy of living cells in culture ; pt. A)
Academic Press, c1989
- : comb bound
- : casebound
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注記
Includes bibliographies and index
内容説明・目次
内容説明
Parts A and B, companion volumes on fluorescence microscopy, are sourcebooks of methods for living cells. Part A comprehensively covers methodology: fluorescent analogs, procedures for fluorescent labeling of various cellular structures, and fluorescence microscopy. Part B describes instrumentation and quantitation analysis for imaging and spectroscopy.
目次
Fluorescent Analog Cytochemistry: Tracing Functional Protein Components in Living Cells. Fluorescent Analogs of Peptides and Hormones. Fluorescent Analogs of Toxins. Preparation of Fluorescently Labeled Dextrans and Ficolls. A Fluorescent Derivative of Ceramide: Physical Properties and Use in Studying the Golgi Apparatus of Animal Cells. Fluorescent Labeling of Cell Surfaces. Fluorescent Labeling of Mitochondria. Fluorescent Labeling of Endoplasmic Reticulum. Fluorescent Labeling of Endocytic Compartments. Incorporation of Macromolecules into Living Cells. Hapten-Mediated Immunocytochemistry: The Use of Fluorescent and Nonfluorescent Haptens for the Study of Cytoskeletal Dynamics in Living Cells. Culturing Cells on the Microscope Stage. Basic Fluorescence Microscopy. Quantitative Fluorescence Microscopy Using Photomultiplier Tubes and Imaging Detectors. Characteristics of Low Light Level Television Cameras. Solid-State Imagers for Microscopy. Each chapter includes references. Index. Contents of Recent Volumes.
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