High performance liquid chromatography
Author(s)
Bibliographic Information
High performance liquid chromatography
（Analytical chemistry by open learning）
Published on behalf of ACOL, London by Wiley, c1987
- : pbk.
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Note
Bibliography: p. xvii-xviii
Description and Table of Contents
Description
HPLC is the most powerful of all chromatographic techniques but because of its sophistication, it requires both extensive experience and good theoretical knowledge. The book provides practical experience in the use of basic HPLC equipment, demonstrates the various parameters that control HPLC equipment, demonstrates the various parameters that control HPLC separations, shows the use of the technique for separation and quantitative analysis and illustrates some of the important principles from the theory part of the unit. All the background to the technique, detail on available instrumentation and on appreciation of results interpretation and the effect of varying instrumental parameters are covered.
Table of Contents
- Introduction
- Instrumentation: The Basic Component of a High Performance Liquid Chromatography
- Pumps and Injection Systems
- Columns
- Detectors
- Column Packings and Modes of HPLC: Bonded Phase Chromatography
- The Chromatography of Ionic Solutes
- Adsorption and Exclusion Chromatography
- Some Applications of HPLC: Factors Affecting Resolution
- Developing a Separation
- Gradient Elution
- Quantitative Analysis
- Some Practical Aspects of HPLC: Packing Columns
- The Preparation of Mobile Phases
- Practical Procedures with Columns and Samples
- Self Assessment Questions and Responses
- Units of Measurement.
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