Neurochemistry : a practical approach
著者
書誌事項
Neurochemistry : a practical approach
（The practical approach series）
IRL, c1987
- : hard
- : pbk.
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注記
Includes bibliographical references and index
内容説明・目次
- 巻冊次
-
: pbk. ISBN 9781852210274
内容説明
Neurobiology has undergone a revolution with many advances dependent on new techniques and approaches. This fully updated second edition reflects the recent growth areas in this subject. Its major themes are the fractionation and study of cellular and subcellular components of the nervoussystem, chemical neuroanatomy, and the measurement of neuronal metabolism in vivo and in vitro. Topics include the neuronal cytoskeleton; isolation of synaptosomes and their subcellular components; aspects of mitochondrial defects in neurones; and synaptic transport processes exemplified by the GABAtransporter. This is an invaluable practical laboratory manual for all researchers in neurobiology; it contains step-by-step protocols and a troubleshooting guide, as well as examples of experimental data and their interpretation.
目次
- Abbreviations
- Isolation of synaptosomes
- growth clones and their subcellular components
- Neuronal and glial cells: cell culture of the central nervous system
- Immunocytochemical techniques for the localization of neurochemically characterized nerve pathways
- The use of bioluminescence techniques in neurobiology, with emphasis on the cholinergic system
- Neuroreceptors
- Second messengers and protein phosphorylation in the nervous system
- Physiological responses to receptor activation: phosphoinositide turnover
- Isolation procedures and in vitro applications of cell nuclei from the mammalian brain
- Functional expression in the xenopus oocyte of mRNAs for receptors and ion channels
- 巻冊次
-
: hard ISBN 9781852210281
内容説明
Neurobiology has undergone a revolution with many advances dependent on new techniques and approaches. This fully updated second edition reflects the recent growth areas in this subject. Its major themes are the fractionation and study of cellular and subcellular components of the nervoussystem, chemical neuroanatomy, and the measurement of neuronal metabolism in vivo and in vitro. Topics include the neuronal cytoskeleton; isolation of synaptosomes and their subcellular components; aspects of mitochondrial defects in neurones; and synaptic transport processes exemplified by the GABAtransporter. This is an invaluable practical laboratory manual for all researchers in neurobiology; it contains step-by-step protocols and a troubleshooting guide, as well as examples of experimental data and their interpretation.
目次
- P.R. Gordon-Weeks: Isolation of synaptosomes, growth clones and their subcellular components
- R.P. Saneto and J de Vellis: Neuronal and glial cells: cell culture of the central nervous system
- J.V. Priestley: Immunocytochemical techniques for the localization of neurochemically characterized nerve pathways
- M. Israel and B. Lesbats: The use of bioluminescence techniques in neurobiology, with emphasis on the cholinergic system
- G.G. Lunt: Neuroreceptors
- H.C. Palfrey and P. Mobley: Second messengers and protein phosphorylation in the nervous system
- C.M.F. Simpson, I.H. Batty, and J.N. Hawthorne: Physiological responses to receptor activation: phosphoinositide turnover
- R.J. Thompson: Isolation procedures and in vitro applications of cell nuclei from the mammalian brain
- E.A. Barnard and G. Bilbe: Functional expression in the xenopus oocyte of mRNAs for receptors and ion channels
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