Lymphokines and interferons : a practical approach
著者
書誌事項
Lymphokines and interferons : a practical approach
（The practical approach series）
IRL Press, c1987
- : pbk
- : hbk
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注記
Includes bibliographical references and index
内容説明・目次
- 巻冊次
-
: pbk ISBN 9781852210359
目次
- Induction, production and purification of natural mouse IFN-a and b
- Induction, production and purification of murine gamma interferon
- Production and purification of recombinant mouse interferon -y from E.Coli
- Production of human interferon -a
- Production of recombinant interferons by expression in heterologous mammalian cells
- Biological assays for interferons
- Quantitation of IFN mRNA
- Antibodies against interferons: characterization of interferos and immunoassays
- Quantification of interferons by anti-viral assays and their standardization
- Analysis of anti-viral mechanisms: interferon-regulated 2'5' oligoadenylate and protein kinase systems
- Cell growth inhibition by interferons and tumour necrosis factor
- Cytoxicity assays for tumour necrosis factor and lymphotoxin
- Anti-microbial and hydrogen peroxide assays for mafs
- In vitro tumour cytotoxicity assays for macrophage activation
- Measurement of interleukin-1 activity
- Production and assay of interleukin 2
- Assays for interleukin 3 and other myeloid colony-stimulating factors
- Eosinophil differentiation factor and its associated B cell growth factor activities
- Assays for human B cell growth and differentiation factors
- 巻冊次
-
: hbk ISBN 9781852210366
内容説明
A practical response to the increase of interest in the fields of interferon and lymphokine research, this volume presents the techniques involved with these independent areas of research. Reflecting recent progress in these fields, contributors describe protocols for working with interferons including antibody preparation, immuno-assay, purification, expressing recombinant genes in heterologous systmes and quantitating mRNA with cDNA probes; the chapters on techniques for lymphokines address aspects unique to each different lymphokine. Readership: immunology, biology researchers, postgraduates.
目次
- Induction, production and purification of natural mouse IFN-a and b
- Induction, production and purification of murine gamma interferon
- Production and purification of recombinant mouse interferon -y from E.Coli
- Production of human interferon -a
- Production of recombinant interferons by expression in heterologous mammalian cells
- Biological assays for interferons
- Quantitation of IFN mRNA
- Antibodies against interferons: characterization of interferos and immunoassays
- Quantification of interferons by anti-viral assays and their standardization
- Analysis of anti-viral mechanisms: interferon-regulated 2'5' oligoadenylate and protein kinase systems
- Cell growth inhibition by interferons and tumour necrosis factor
- Cytoxicity assays for tumour necrosis factor and lymphotoxin
- Anti-microbial and hydrogen peroxide assays for mafs
- In vitro tumour cytotoxicity assays for macrophage activation
- Measurement of interleukin-1 activity
- Production and assay of interleukin 2
- Assays for interleukin 3 and other myeloid colony-stimulating factors
- Eosinophil differentiation factor and its associated B cell growth factor activities
- Assays for human B cell growth and differentiation factors
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