Protein sequencing : a practical approach
著者
書誌事項
Protein sequencing : a practical approach
（The practical approach series）
IRL Press, c1989
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注記
Includes bibliographies and index
内容説明・目次
- 巻冊次
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ISBN 9780199630127
内容説明
The ability to determine the primary structure of a polypeptide was a major advance in biochemistry and sequencing work since then has let to a greater understanding of protein structure and function. At present, protein sequence analysis is at the forefront of modern molecular biology. Partial sequence determinations using microgramme amounts of protein has enabled many genes with low levels of transcription to be cloned and their complete primary structures to be determined. This approach is also the only means available for determining the sites of post-translational modification. This is a laboratory manual aimed at those wishing to isolate proteins or peptides and to carry out subsequent sequence analysis and interpretations of structural significance. It is written for both professional and inexperienced research workers and for those with and without access to high technology instrumentation. The protocols used have been selected for their general utility.
目次
- Protein and peptide purification, K.J.Wilson and P.M.Yuan
- peptide preparation and characterization, A.Aitken et al
- automated solid phase microsequencing, J.B.C.Findlay and D.J.C.Pappin and J.N.Keen
- gas or pulsed-liquid phase sequence analysis, M.J.Geishow and A.Aitken
- sequencing by mass spectrometry, K.Biemann
- manual methods of protein sequencing, A.Yarwood
- structure prediction, J.Thorton and W.R.Taylor.
- 巻冊次
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ISBN 9780199630134
内容説明
The ability to determine the primary structure of a polypeptide was a major advance in biochemistry and sequencing work since then has let to a greater understanding of protein structure and function. At present, protein sequence analysis is at the forefront of modern molecular biology. Partial sequence determinations using microgramme amounts of protein has enabled many genes with low levels of transcription to be cloned and their complete primary structures to be determined. This approach is also the only means available for determining the sites of post-translational modification. This is a laboratory manual aimed at those wishing to isolate proteins or peptides and to carry out subsequent sequence analysis and interpretations of structural significance. It is written for both professional and inexperienced research workers and for those with and without access to high technology instrumentation. The protocols used have been selected for their general utility.
目次
- K. J. Wilson & P. M. Yuan: Protein and peptide purification
- A. Aitken, M. J. Geisow, J.B.C. Findlay, C. Holmes & A. Yarwood: Peptide preparation and characterisation
- J.B.C. Findlay, D.J.C. Pappin & J.N. Keen: Automated solid phase microsequencing
- M.J. Geisow & A. Aitken: Gas or pulsed-liquid phase sequence analysis
- K. Biemann: Sequencing by mass spectrometry
- A. Yarwood: Manual methods of protein sequencing
- J. Thorton & W.R. Taylor: Structure prediction
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