Immunochemical and molecular genetic analysis of bacterial pathogens
Author(s)
Bibliographic Information
Immunochemical and molecular genetic analysis of bacterial pathogens
（FEMS symposium, no. 40）
Elsevier, 1988
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"Based on the proceedings of a laboratory workshop held under the auspices of the Federation of European Microbiological Societies and the Society of General Microbiology in Dublin, Ireland from 20 July-2 August 1986"--T.p. verso
Includes bibliographies and index
Description and Table of Contents
Description
This book is based on the FEMS/SGM-sponsored laboratory course `Immunochemical and Molecular Genetic Analysis of Bacterial Pathogens and their Virulence Determinants'. The volume deals, in review form, with the contribution made to virulence by individual surface structures and toxins. Later chapters detail methodology related to the cloning of virulence genes and to the purification, assay and immuno/biochemical analysis of their products. In producing this volume recognition has been made of the fact that research scientists need, as two of their basic requirements, a series of tested protocols which may be readily applied to the problem at hand, and topical reviews which succinctly summarize progress in the field. Immunochemical and Molecular Genetic Analysis of Bacterial Pathogens is a companion to the 1985 Elsevier Publication `Enterobacterial Surface Antigens: Methods for Molecular Characterization' (Korhonen, T.K., Dawes, E.A., and Makela, P.H., eds.) also based on a related FEMS laboratory course.
Table of Contents
Preface. List of contributors. TOPICAL REVIEW SECTION Chapter 1: Flagella: their role in virulence (C.J. Smyth). Chapter 2: Fimbriae: their role in virulence (C.J. Smyth). Chapter 3: Outer membrane proteins: their role in virulence (P. Owen). Chapter 4: Extracellular toxins: their role in virulence (J.P. Arbuthnott). Chapter 5: Surface antigens of Bordetella pertussis: their role in virulence (J.H. Freer). Chapter 6: Genetic analysis of pathogenesis of enteric bacteria (R. Curtiss III, A.T. Maurelli and P.A. Gulig). Chapter 7: Cholera, the cholera enterotoxins, and the cholera enterotoxin-related enterotoxin family (R.A. Finkelstein). Chapter 8: Genetic analysis of Staphylococcus aureus virulence (T.J. Foster, P.W. O'Toole and M. O'Reilly). Chapter 9: Analysis of virulence of Treponema pallidum: the pathology and immunology of experimental syphilis (M.J. Bailey, A. Cockayne and C.W. Penn). Chapter 10: Surface antigens of Pseudomonas aeruginosa: analysis by crossed immunoelectrophoresis and role in protection (S. MacIntyre, T. McVeigh and P. Owen). Chapter 11: Strategies in bacterial vaccine development (D.J. Maskell and G. Dougan). Chapter 12: Animal models in the study of pathogenesis (C. Adlam). METHODS SECTION Chapter 13: Construction of gene libraries in bacteriophage lambda vectors (P. O'Toole and T.J. Foster). Chapter 14: Screening lambda libraries and detection of recombinants (M. O'Reilly, P. O'Toole and T.J. Foster). Chapter 15: Transformation of bacterial protoplasts (M. O'Reilly and T.J. Forster). Chapter 16: Production of monoclonal antibodies for studies of bacterial virulence (A. McHale and R.J. Russell). Chapter 17: Assays for fimbrial adhesins (C.J. Smyth). Chapter 18: Crossed immunoelectrophoresis: a literature guide (P. Owen). Chapter 19: Western immunoblotting (P. Caffrey, T. McVeigh and P. Owen). Chapter 20: Silver staining of proteins and lipopolysaccharides in SDS-polyacrylamide gels (T. McVeigh, P. Caffrey and P. Owen). Chapter 21: Purification of Staphylococcal exotoxins (J.C.S. De Azavedo and J.P. Arbuthnott). Chapter 22: Detection of extracellular toxins (J.C.S. De Azavedo and J.P. Arbuthnott).Chapter 23: An in vitro method for the study of leucocyte migration inhibition (R.J. Russell). Chapter 24: A simple method of bacterial characteristics and growth in vitro (J.C.S. De Azavedo and R.J. Russell). Chapter 25: Animal requirements in the study of bacterial pathogenesis (P. Nowlan). Subject Index.
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