Selective sample handling and detection in high-performance liquid chromatography
著者
書誌事項
Selective sample handling and detection in high-performance liquid chromatography
（Journal of chromatography library, v. 39）
Elsevier, 1988-1989
- pt. A
- pt. B
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注記
Includes bibliographies and index
内容説明・目次
- 巻冊次
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pt. A ISBN 9780444428813
内容説明
This book is the first of a two-volume project which attempts to treat the sample handling and detection processes in a liquid chromatographic system in an integrated fashion. The need for more selective and sensitive chromatographic methods to help solve the numerous trace analysis problems in complex samples is undisputed. However, few workers realize the strong interdependence of the various steps - sample handling, separation and detection - which must be considered if one wants to arrive at an optimal solution. By introducing a strong element of selectivity and trace enrichment in the sample preparation step, fewer demands are placed on the quality of the chromatography and often a simple UV detector can be used. By using a selective detection mode, i.e. a reaction detector, the sample handling step can frequently be simplified and more easily automated. The impact of such a ``total system'' approach on handling series of highly complex samples such as environmental specimens or biological fluids can be easily imagined. Each chapter includes sufficient references to the literature to serve as a valuable starting point for more detailed investigation.
目次
1. On-line sample handling and trace enrichment in liquid chromatography. The determination of organic compounds in water samples (M.W.F. Nielen et al.). Instrumentation. On-line sample handling and trace enrichment. Miniaturization of pre-column technology. Conclusion and further developments. 2. Determination of drugs and their metabolites in biological samples by fully automated HPLC with on-line solid-liquid extraction and pre-column switching (R. Huber, K. Zech). Sample preparation techniques. Pre-column technique with column switching. Analytical separation system. Applications. 3. Immobilization of compounds for selective interaction with analytes in liquid chromatography (G. Gubitz). Surface modification of siliceous supports. Chemically modified phases for sample pretreatment and selective separation. Chemically modified supports for selective detection. 4. Design and choice of suitable labelling reagents for liquid chromatography (K. Imai, T. Toyo'oka). Derivatization for UV or visible detection. Derivatization for fluorescence detection. Derivatization for electrochemical detection (ED). Derivatization for chemiluminescence (CL) detection. 5. Photodiode array detection and solute recognition in high-performance liquid chromatography (A.F. Fell, B.J. Clark). Digital methods. Development trends. 6. Electrochemical techniques for detection in HPLC (W.Th. Kok). LCEC: State of the art. Amperometric detection. Dual electrode systems. Derivatization for LCEC. Active metal electrodes. On-line coulometric titrations. Electrochemical signal enhancement. 7. Solid-phase reactors in high-performance liquid chromatography (L. Nondek, R.W. Frei). Solid-phase derivatization reactions. The use of tubular reactors for solid-phase derivatizations. Practical applications of SPRs in HPLC. 8. Commercial aspects of post-column reaction detectors for liquid chromatography (R. Weinberger, R.A. Femia). Instrumentation. Applications based systems. How to optimize a post-column chemistry. Prospective. Manufacturers' directory. Subject Index.
- 巻冊次
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pt. B ISBN 9780444883278
内容説明
This is the second of a two-volume project which treats the handling, separation and detection of complex samples as an integrated, interconnected process. On the basis of this philosophy the editors have selected those contributions which demonstrate that optimal sample preparation leads to a simplification of detection or reduced demands on the separation process. Throughout the book emphasis is on chemical principles with minimum discussion of the equipment required - an approach which reflects the editors' view that the limiting factor in the analysis of complex samples is an incomplete knowledge of the underlying chemistry rather than the hardware available. This lack of knowledge becomes more evident as the demands for lower detection limits grow, as solving complex matrix problems requires a greater understanding of the chemical interaction between the substance to be analysed and the stationary phase. Thus, apart from one chapter dealing with chemically modified silicas, the main theme of the book is developed in three chapters on sample preparation and three on detection.
目次
I. Preconcentration and Chromatography on Chemically Modified Silicas with Complexation Properties (J.L. Veuthey, M.A. Bagnoud, W. Haerdi). II. Sample Handling in Ion Chromatography (P.R. Haddad). III. Whole Blood Sample Clean-Up for Chromatographic Analysis (U. Christians, K.-Fr. Sewing). IV. Radio-Column Liquid Chromatography (A.C. Veltkamp). V. Modern Post-Column Reaction Detection in High-Performance Liquid Chromatography (H. Jansen and R.W. Frei). VI. New Luminescence Detection Techniques (C. Gooijer, N.H. Velthorst, R.W. Frei). VII. Continuous Separation Techniques in Flow-Injection Analysis (M. Valcarcel, M.D. Luque de Castro). Subject Index.
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