Plant protoplasts and genetic engineering

Author(s)

• Bajaj, Y. P. S.

Bibliographic Information

Plant protoplasts and genetic engineering

edited by Y.P.S. Bajaj

（Biotechnology in agriculture and forestry, 8-9, 22-23, 29, 34, 38）

Springer-Verlag, c1989-

• v. 1 : Germany
• v. 1 : U.S.
• v. 2 : Germany
• v. 2 : U.S.
• v. 3 : Germany
• v. 3 : U.S.
• v. 4 : Germany
• v. 4 : U.S.
• v. 5 : Germany
• v. 5 : U.S.
• v. 6 : Germany
• v. 6 : U.S.
• v. 7

Note

Includes bibliographies and index

Description and Table of Contents

Volume

v. 1 : Germany ISBN 9783540191940

Description

Isolated protoplasts are a unique tool for genetic manipulation of plants. Since the discovery of a method for the enzymatic isolation of pro-. toplasts by Professor E. C. Cocking in 1960, tremendous progress has been made in this very fascinating area of research. I have witnessed the struggle in the 1960's and early 1970's, when obtaining a clean prepara tion of protoplasts was considered an achievement. I also shared the pioneering excitement and enthusiasm in this field during the 2nd Inter national Congress of Plant Tissue Culture held at Strasbourg in 1970, where Dr. I. Thkebe of Japan presented his work on the induction of division in tobacco protoplasts. This was followed by my participation in three international conferences devoted to plant protoplasts held in 1972 in Salamanca (Spain) and Versailles (France), and then in 1975 in Nottingham (England). The enthusiasm shown by plant scientists at these meetings was ample proof of the bright future of protoplast technology, and it became evident that protoplasts would playa major role in plant biotechnology, especially in genetic engineering. Since then we have never looked back, and now the methods for isolation, fusion, and culture, as well as regeneration of somatic hybrids, have become routine laboratory procedures for most plant species. Currently the focus is on cereal and tree protoplasts. In order to bring about any genetic manipulation through fusion, in corporation of DNA, and transformation, the regeneration of the entire plant through manipulation of protoplasts is a prerequisite."

Volume

v. 2 : Germany ISBN 9783540507895

Table of Contents

I Genetic Engineering and In Vitro Manipulation of Plant Cells - Technical Advances.- 1 Introduction.- 2 Somatic Hybrids and Cybrids.- 3 Recombinant DNA Technology and Transformation.- 4 Uptake and Incorporation of Organelles, Viruses, Bacteria, and Fungi by Protoplasts.- 5 Newer Techniques for Genetic Engineering.- 6 Conclusions.- References.- II Genetic Manipulation: Generative Versus Somatic.- 1 Introduction.- 2 The Structure of a Plant Breeding Program.- 3 Construction and Isolation of a Desired Genotype.- 4 Reproductive Systems.- 5 Conclusions and Prospects.- References.- III Uptake and Integration of Exogenous DNA in Plants.- 1 Introduction.- 2 A Brief History of Plant Genetic Transformation.- 3 Uptake of Purified DNA by Seeds, Seedlings, and Plant Cells.- 4 Uptake of Purified DNA by Gametes.- 5 Uptake of Purified DNA by Protoplasts.- 6 Nature of Expression Vectors Used in Direct Gene Transfer.- 7 Integration of Foreign DNA Taken up by Protoplasts.- 8 Present Status and Conclusions.- References.- IV DNA Recombinants and Transformation of Agricultural Crops.- 1 Introduction.- 2 The Agrobacterium System.- 3 Vector Systems.- 4 Transfer and Expression of Foreign DNA in Plant Cells.- 5 Use of Selectable Reporter Genes in Plant Cells.- 6 Plant Transformation Systems.- 7 Use of Reverse Genetics Techniques to Study Plant Gene Regulation.- 8 Variability of Transformed Plants.- 9 Conclusions.- References.- V Genetically Transformed Maize (Zea mays L.) Plants from Protoplasts.- 1 Introduction.- 2 Methodology and Experimental Results.- 3 Summary and Conclusions.- References.- VI Protoplast Culture and Transformation Studies on Rice.- 1 Introduction.- 2 Isolation and Culture of Rice Protoplasts.- 3 Recent Progress in Culture and Regeneration of Rice Protoplasts (1984-1987).- 4 Transformation of Rice Protoplasts by Spheroplast Treatment.- 5 Introduction and Expression of Foreign Genes in Gramineae Plants.- 6 Summary and Conclusion.- References.- VII Transformation in Potato.- 1 Introduction.- 2 Transformation Studies in Potato for Resistance to Phytophthora infestans.- 3 Techniques and Material Used for Transformation Studies.- 4 Results.- 5 Other Studies on Transformation in Potato.- 6 Significance of the Work and Its Prospects.- References.- VIII Transformation in Cotton.- 1 General Account.- 2 Transformation and Plant Regeneration in Cotton.- 3 Conclusions and Prospects.- References.- IX Transformation in Populus spp..- 1 Introduction.- 2 Genetic Transformation of Populus.- 3 Conclusions.- References.- X Transformation in Vinca rosea L.: An Example of Spheroplast Method for Gene Transfer.- 1 Introduction.- 2 Techniques.- 3 Technical Problems in the Spheroplast Method.- 4 Mechanism of Introduction of Spheroplasts into Protoplasts.- 5 Introduction of E. coli Spheroplasts into Protoplasts.- 6 Summary and Conclusion.- References.- XI Transformation in Hyoscyamus.- 1 Introduction.- 2 In Vitro Transformation of Protoplast-Derived H. muticus Cells by A. tumefaciens.- 3 Summary and Conclusions.- References.- XII Genetic Manipulation in Brassica.- 1 Introduction.- 2 Protoplast Fusion.- 3 Transformation.- 4 Conclusion.- References.- XIII Direct Gene Transfer in Protoplasts of Nicotiana plumbaginifolia.- 1 Introduction.- 2 A Simple Chemical Procedure for the Stable Transformation of Nicotiana plumbaginifolia Protoplasts.- 3 Stable Transformation of Nicotiana plumbaginifolia by Electroporation.- 4 Transient Expression of a Foreign Gene Introduced by a PEG Treatment into Nicotiana plumbaginifolia Protoplasts.- 5 Discussion.- References.- XIV Microinjection of Cells and Protoplasts: Integration of Foreign DNA.- 1 Introduction.- 2 Techniques.- 3 Uptake and Integration of DNA.- 4 Future Directions.- 5 Protocol.- References.- XV Electrofusion: The Technique and Its Application to Somatic Hybridization.- 1 Introduction.- 2 Biophysics of Electrofusion.- 3 Application of Electrofusion to Plant Protoplasts.- 4 Conclusions and Prospects.- References.- XVI Versatile Apparatuses for Electrogene Mapping, Electrophoresis and Electrofusion.- 1 Introduction.- 2 Fundamental Theory and Physical Parameters in Electrophoresis.- 3 Versatile Apparatus for Electrophoresis Including Isoelectrofocusing.- 4 Highly Simplified Horizontal Electrophoretic Apparatus Including a Simple Power Supply.- 5 Protoplast Fusion by Electric Impulse Generated by the Simple Power Supply.- 6 Construction of a Versatile Apparatus for PFGE and Its Possible Application to "Electrogene Mapping".- References.- XVII Chloroplast Genomes as Genetic Markers.- 1 Introduction.- 2 Chloroplast Genome and Its Properties.- 3 Chloroplast Genomes and Their Application as Genetic Markers.- 4 Conclusions.- References.- XVIII Flow Cytometric Analysis and Sorting of Somatic Hybrid and Transformed Protoplasts.- 1 Introduction.- 2 Flow Analysis and Sorting of Somatic Hybrid Protoplasts.- 3 Flow Analysis and Sorting of Transformed Protoplasts.- 4 Summary and Conclusions.- References.- XIX Isolation of Nuclei and Their Transplantation into Plant Protoplasts.- 1 Introduction.- 2 Sources of Nuclei.- 3 Isolation Techniques.- 4 Isolation and Purification of Nuclei.- 5 Induction of Uptake of Nuclei by Protoplasts.- 6 Post Transplantation Events.- 7 Concluding Remarks.- 8 The Protocol for Isolation and Transplantation of Nuclei.- References.- XX Chromosome Transplantation and Applications of Flow Cytometry in Plants.- 1 Introduction.- 2 Flow Cytometry and Its Application.- 3 Chromosome Isolation and Sorting.- 4 Characterization of Isolated, Flow-Sorted Chromosomes.- 5 Chromosome Transfer.- 6 Conclusions.- References.- XXI Transfer of Mitochondria Through Protoplast Fusion.- 1 Introduction.- 2 Selection Method of Formation of Daucus Cybrid Plants by Donor-Recipient Protoplast Fusion.- 3 Characterization of Putative Cybrid Plants.- 4 Restriction and Southern Analysis of mtDNA's from Carrot Cybrids.- 5 Conclusion.- References.- XXII Incorporation of the Firefly Luciferase Gene into Plant Cells.- 1 Introduction.- 2 The Firefly Luciferase.- 3 Expression of the CaMV 35S-Luciferase Chimeric Gene.- 4 Spatial Pattern of Gene Expression.- 5 Expression of Luciferase in Cultured Cells.- 6 Transient Expression Studies.- 7 Conclusions and Prospects.- 8 Protocol.- References.- XXIII Uptake of Viruses by Plant Protoplasts and Their Use as Transforming Agents.- 1 Introduction.- 2 Chemical Methods for the Inoculation of Plant Protoplasts.- 3 Electrical and Physicochemical Methods for the Inoculation of Plant Protoplasts.- 4 Liposome Carriers for the Inoculation of Protoplasts.- 5 Efficiency of Inoculation Procedure.- 6 Virus Uptake and Transformation.- 7 Summary and Conclusions.- References.- XXIV Uptake of Fungal Protoplasts by Plant Protoplasts.- 1 Introduction.- 2 Protoplast Isolation.- 3 Initiation of Fusion Between Plant and Fungal Protoplasts.- 4 Prolonged Incubation of Plant-Fungal Protoplasts.- 5 The Fate of Plant-Fungal Protoplasts.- 6 Conclusions.- 7 Summary Protocol.- References.- XXV Chloroplast Uptake and Genetic Complementation.- 1 Chloroplast Uptake.- 2 The Preparation of Material.- 3 Genetic Complementation.- 4 A Summary - The Agronomic Potentials of Chloroplast Uptake.- 5 The Potential for the Uptake of Blue Green Algae.- 6 Conclusions and Prospects.- References.- XXVI Cytoplasts: Isolation and Uses.- 1 Introduction.- 2 Cytoplasts.- 3 Uses of Cytoplasts.- 4 Conclusions and Prospects.- References.- XXVII Vacuoles: Isolation, Purification, and Uses.- 1 Introduction.- 2 General Methods of Isolation.- 3 Purification Procedures.- 4 Isolation and Purification Scheme.- 5 Uses of Vacuoles.- 6 Conclusions.- References.- XXVIII Miniprotoplasts and Their Use in Genetic Engineering.- 1 Introduction.- 2 Types of Subprotoplasts.- 3 Methods for Fragmentation of Protoplasts.- 4 Characterization of Miniprotoplasts.- 5 Use and Significance of Miniprotoplasts in Cell Manipulation and Genetic Engineering.- 6 Summary and Conclusions.- References.

Volume

v. 3 : Germany ISBN 9783540563136

Description

In continuation of Volumes 8 and 9 (1989) on in vitromanipulation of plant protoplasts, this new volume dealswith the regeneration of plants from protoplasts and genetictransformation in various species of Agrostis, Arabidopsis,Atropa, Brassica, Catharanthus, Datura, Cucumis, Daucus,Digitalis, Duboisia, Eustoma, Festuca, Helianthus, Hordeum,Kalanchoe, Linum, Lobelia, Lolium, Lotus, Lycium,Lycopersicum,Mentha, Nicotiana, Pelargonium, Pisum, Pyrus,Salvia, Scopolia, and Solanum.These studies reflect the farreaching implications of protoplast technologyin geneticengineering of plants. They are of special interest toresearchers in the field of plant tissue culture, molecularbiology, genetic engineering, and plant breeding.

Table of Contents

Section I Regeneration of Plants from Protoplasts.- I.1 Regeneration of Plants from Protoplasts of Agrostis alba (Redtop).- 1 Introduction.- 2 Induction of Embryogenic Callus and Establishment of Embryogenic Suspension Culture.- 3 Isolation of Protoplasts.- 4 Culture of Protoplasts.- 5 Regeneration of Plants.- 6 Conclusions.- 7 Protocol.- References.- I.2 Regeneration of Plants from Protoplasts of Atropa belladonna L. (Deadly Nightshade).- 1 Introduction.- 2 Isolation and Culture of Protoplasts.- 3 Protoplast Fusion and Somatic Hybridization.- 4 Cryopreservation of Protoplasts.- 5 Summary.- 6 Protocol.- References.- I.3 Regeneration of Plants from Protoplasts of Brassica alboglabra Bailey (Chinese Kale).- 1 Introduction.- 2 Isolation of Protoplasts.- 3 Culture of Protoplasts.- 4 Regeneration and Acclimatization of Plants.- 5 Characterization of Protoplast-Derived Plants.- 6 Gene Transfer into Protoplasts Using Electroporation.- 7 Conclusions and Prospects.- 8 Protocol.- References.- I.4 Regeneration of Plants from Protoplasts of Cucumis sativus L. (Cucumber).- 1 Introduction.- 2 Isolation of Protoplasts.- 3 Culture of Protoplasts.- 4 Regeneration of Plants.- 5 Fusion of Protoplasts.- 6 Conclusions and Prospects.- 7 Protocol for Isolation, Culture, and Regeneration of Cucumber Protoplasts.- References.- I.5 Regeneration of Plants from Protoplasts of Lolium (Ryegrasses) and Festuca (Fescues).- 1 Introduction.- 2 Isolation of Protoplasts.- 3 Culture of Protoplasts.- 4 Culture Media and Conditions.- 5 Regeneration from Protoplast Colonies.- 6 Somaclonal Variation in Regenerated Plants.- 7 Summary and Conclusions.- 8 Protocol.- References.- I.6 Regeneration of Plants from Protoplasts of Lotus spp. (Birdsfoot trefoil).- 1 Introduction.- 2 Protoplast Isolation.- 3 Protoplast Culture and Plant Regeneration.- 4 Conclusion and Prospects.- 5 Protocol.- References.- I.7 Regeneration of Plants from Protoplasts of Lycium barbarum L. (Wolfberry).- 1 Introduction.- 2 Plant Material and Callus Culture.- 3 Mesophyll and Callus Protoplast Isolation.- 4 Mesophyll Protoplast Culture and Plant Regeneration.- 5 Culture of Callus-Derived Protoplasts.- 6 Summary and Conclusions.- References.- I.8 Regeneration of Plants from Protoplasts of Pelargonium spp. (Geranium).- 1 Introduction.- 2 Isolation of Protoplasts.- 3 Culture of Protoplasts.- 4 Regeneration of Plants.- 5 Plastid Inheritance.- 6 Conclusion.- 7 Protocols.- References.- I.9 Regeneration of Plants from Protoplasts of Pea (Pisum sativum L.).- 1 Introduction.- 2 Isolation of Protoplasts.- 3 Protoplast Culture.- 4 Regeneration of Plants.- 5 Protocol.- References.- I.10 Regeneration of Plants from Protoplasts of Pyrus spp. (Pear).- 1 Introduction.- 2 Isolation of Protoplasts.- 3 Culture of Protoplasts.- 4 Plant Regeneration from Protoplast-Derived Callus.- 5 Protoplast Fusion and Somatic Hybridization Studies with Pyrus Protoplasts.- 6 Miscellaneous Studies with Pyrus Protoplasts.- 7 Summary and Conclusion.- 8 General Protocols.- References.- Section II Genetic Transformation.- II.1 Transformation in Arabidopsis thaliana.- 1 Introduction.- 2 Plant Culture and Protoplast Isolation.- 3 Direct Gene Transfer to Protoplasts.- 4 Selection of Transformed Colonies.- 5 Plant Regeneration.- 6 Concluding Remarks.- References.- II.2 Transformation in Atropa belladonna.- 1 Introduction.- 2 Preparation of Agrobacterium.- 3 Transformation.- 4 Elimination of Bacteria and Selection of Transformed Cells.- 5 Regeneration of Plants.- 6 Transgenic A. belladonna.- 7 Conclusion.- 8 Protocols for Transformation.- References.- II.3 Transformation in Catharanthus Species (Madagascar Periwinkle).- 1 Introduction.- 2 Importance of Catharanthus.- 3 Genetic Transformation of Catharanthus Species.- 4 Potential of Hairy Root Cultures.- 5 Transformation of C. roseus and Plant Pathology.- 6 Genetic Engineering with Genes Involved in Tropane Alkaloids Synthesis.- 7 Conclusion.- References.- II.4 Transformation in Datura Species.- 1 Introduction.- 2 Tropane Alkaloid Production.- 3 Transformation.- 4 Summary and Conclusion.- References.- II.5 Transformation in Daucus carota L. (Carrot).- 1 Introduction.- 2 Stable Genetic Transformation of Carrot.- 3 Applications of Electroporation of Carrot Protoplasts.- 4 Isolation and Culture of Carrot Protoplasts.- 5 Electroporation Parameters.- 6 Protocol for the Establishment of an Electroporation System.- 7 Conclusion.- References.- II.6 Transformation in Digitalis purpurea L. (Foxglove).- 1 Introduction.- 2 Transformation.- 3 Expression of Chimeric Genes in Transgenic Digitalis Roots.- 4 Production of Cardioactive Glycosides.- 5 Summary and Conclusions.- 6 Protocols.- References.- II.7 Transformation in Duboisia spp..- 1 Introduction.- 2 Genetic Transformation in Duboisia by the Ri T-DNA of Agrobacterium rhizogenes.- 3 Somatic Hybrids Between Duboisia and Nicotiana by Protoplast Fusion.- 4 Summary and Conclusions.- References.- II.8 Transformation in Eustoma grandiflorum (Lisianthus).- 1 Introduction.- 2 Breeding and In Vitro Propagation.- 3 Agrobacterium-Mediated Transformation.- 4 Analysis of Early Transformation Events.- 5 Production of Kanamycin-Resistant Shoots.- 6 Southern Hybridization Analysis.- 7 Future Directions: Manipulation of Color in Lisianthus.- References.- II.9 Transformation in Helianthus annuus L. (Sunflower).- 1 Introduction.- 2 Methodology and Experimental Results.- 3 Summary and Conclusions.- References.- II.10 Transformation in Hordeum vulgare (Barley).- 1 Introduction.- 2 Culture of Barley Coleoptile Tissues.- 3 Transient Expression of Foreign Genes Introduced into Barley Coleoptile Cells by Microinjection.- 4 Assessment of Transformation Strategy with Respect to Disease Resistance.- 5 Promoter Sequences of Barley Responsible for Infection with Powdery Mildew Pathogen.- 6 Conclusion and Prospects.- References.- II.11 Transformation in Kalanchoe.- 1 Introduction.- 2 Transient Gene Expression.- 3 Stable Transformation.- 4 Transformation Protocol.- 5 Perspectives.- References.- II.12 Transformation in Linum usitatissimum L. (Flax).- 1 Introduction.- 2 Agrobacterium-Mediated Flax Transformation.- 3 Genetic Engineering for Flax Crop Improvement.- 4 Summary and Conclusions.- References.- II.13 Transformation in Lobelia inflata.- 1 Introduction.- 2 Establishment of Hairy Root Culture and Production of Secondary Metabolites.- 3 Results and Discussion.- 4 Summary and Conclusions.- References.- II.14 Transformation in Lycopersicon esculentum L. (Tomato).- 1 Introduction.- 2 Parameters Important for the Preparation and Culture of Tomato Protoplasts.- 3 Direct Gene Transfer into Mesophyll Protoplasts of L. esculentum and L. peruvianum by Electroporation.- 4 Conclusion.- References.- II.15 Transformation in Mentha Species (Mint).- 1 Introduction.- 2 Procedures for the Genetic Manipulation of Mentha.- 3 Phenotypes of Mentha Galls Transformed by Different Strains of A. tumefaciens.- 4 Analysis of Transformed Shoot Cultures.- 5 Summary and Conclusions.- References.- II.16 Transformation in Nicotiana edwardsonii.- 1 Introduction.- 2 Leaf Disc Transformation and Regeneration.- 3 Transient Expression of Introduced Genes in Nicotiana edwardsonii.- 4 Conclusion.- References.- II.17 Transformation in Salvia chinensis.- 1 Introduction.- 2 Plant Regeneration System.- 3 Transgenic Plants and Shoots Produced by Agrobacterium-Mediated Transformation.- 4 Protocol.- 5 Conclusions.- References.- II.18 Transformation in Scopolia.- 1 Introduction.- 2 Cell Culture of Scopolia.- 3 Ri Plasmid Transformation and Hairy Root Culture.- 4 Summary and Conclusions.- References.- II.19 Transformation in Solanum melongena L. (Eggplant).- 1 Introduction.- 2 General Transformation Strategies.- 3 Eggplant Transformation by Cocultivation.- 4 Transgenic Somatic Embryos.- 5 Conclusions.- References.

Volume

v. 4 : Germany ISBN 9783540563846

Description

In continuation of Volumes 8, 9, and 22 on in vitromanipulation of plant protplasts, this new volume deals withthe regeneration of plants from protoplasts and genetictransformation in various species of Actinidia, Amoracia, Beta, Brassica, Cicer, Citrus, Cucumis, Duboisia, Fragaria, Glycine, Ipomoea, Lactuca, Lotus, Lycopersicon, Manihot, Medicago, Nicotiana, Petunia, Phaseolus, Pisum, Prunus, Psophocarpus, Saccharum, Solanum, Sorghum, Stylosanthes, andVitis. These studies reflect the far-reaching implicationsof protoplast technology in genetic engineering of plants. They are of special interest to researchers in the field ofplant tissue culture, molecular biology, geneticengineering, and plant breeding.

Table of Contents

Section I Regeneration of Plants from Protoplasts.- I.1 Regeneration of Plants from Protoplasts of Kiwifruit (Actinidia deliciosa).- 1 Introduction.- 2 Isolation and Culture of Protoplasts.- 3 Growth of Regenerated Plants.- 4 Variability in Regenerated Plants.- 5 Conclusions and Prospects.- References.- I.2 Regeneration of Plants from Protoplasts of Duboisia.- 1 Introduction.- 2 Isolation of Protoplasts.- 3 Culture of Protoplasts.- 4 Regeneration of Plants.- 5 Fusion of Protoplasts.- 6 Miscellaneous Study.- 7 Conclusion and Prospects.- 8 Protocol.- References.- I.3 Regeneration of Plants from Protoplasts of Cultivated Strawberry (Fragaria x ananassa) and Wild Strawberry (Fragaria vesca).- 1 Introduction.- 2 Isolation of Protoplasts.- 3 Culture of Protoplasts.- 4 Regeneration of Plants.- 5 Characterization of Protoplast-Derived Plants.- 6 Genetic Transformation in Strawberry.- 7 Conclusions and Prospects.- 8 Protocol.- References.- I.4 Regeneration of Plants from Protoplasts of Sweet Potato (Ipomoea batatas L. Lam.).- 1 Introduction.- 2 Isolation of Protoplasts.- 3 Culture of Protoplasts.- 4 Plant Regeneration from Protoplast-Derived Callus.- 5 Characterization of Protoplast-Derived Plants.- 6 Fusion of Protoplasts.- 7 Summary and Conclusion.- 8 Protocol.- References.- I.5 Regeneration of Plants from Alfalfa (Medicago sativa L.) Protoplasts by Direct Embryogenesis.- 1 Introduction.- 2 Procedures.- 3 Results.- 4 Discussion.- 5 Protocol.- References.- I.6 Regeneration of Plants from Protoplasts of Adsuki Bean (Phaseolus angularis Wight).- 1 Introduction.- 2 Material and Methods.- 3 Results and Discussion.- 4 Summary.- References.- I.7 Regeneration of Plants from Protoplasts of Some Stone Fruits (Prunus spp.).- 1 Introduction.- 2 Isolation of Protoplasts.- 3 Culture of Protoplasts.- 4 Plant Regeneration from Protoplast-Derived Callus.- 5 Protoplast Fusion and Somatic Hybridization Studies with Prunus Genotypes.- 6 Miscellaneous Studies with Prunus Protoplasts.- 7 Conclusions.- 8 References.- I.8 Regeneration of Plants from Protoplasts of Winged Bean (Psophocarpus tetragonolobus L. DC.).- 1 Introduction.- 2 Isolation of Protoplasts.- 3 Protoplast Purification.- 4 Protoplast Culture.- 5 Plant Regeneration from Protoclones.- 6 Conclusion.- 7 Protocol.- References.- I.9 Regeneration of Plants from Protoplasts of Eggplant (Solanum melongena L.).- 1 Introduction.- 2 Isolation of Protoplasts.- 3 Culture of Protoplasts.- 4 Plant Regeneration from Protoplast-Derived Callus.- 5 Fusion of Protoplasts.- 6 Summary and Conclusion.- 7 Protocol.- References.- I.10 Regeneration of Plants from Protoplasts of Sorghum (Sorghum vulgare).- 1 Introduction.- 2 Embryogenie Callus Formation and Establishment of Suspension Culture.- 3 Protoplast Isolation and Culture.- 4 Plant Regeneration.- 5 Protoplast Transformation by PEG-Mediated DNA Transfer.- 6 Conclusion.- 7 Protocol.- References.- Section II Genetic Transformation.- II. 1 Transformation in Horseradish (Armoracia rusticana): Hairy Roots Incited by Agrobacterium rhizogenes Infection.- 1 Introduction.- 2 Varieties of Horseradish.- 3 Induction of Hairy Roots.- 3 Confirmation of Ri Plasmid-Direction Transformation.- 5 Growth of Hairy Roots.- 6 Bioreactor for Hairy Root Culture.- 7 Peroxidase Productivity of Hairy Roots.- 8 Regeneration of Hairy Roots.- 9 Summary and Conclusion.- References.- II.2 Transformation in Sugar Beet (Beta vulgaris L.).- 1 Introduction.- 2 Transformation by Direct Gene Transfer.- 3 Transformation by Agrobacterium tumefaciens.- 4 Conclusions.- References.- II.3 Transformation in Oilseed Rape (Brassica napus L.).- 1 Introduction.- 2 Susceptibility of Brassica napus to Agrobacterium tumefaciens Infection.- 3 Transformation of Tissue Explants.- 4 Transformation of Protoplasts.- 5 Gene Transfer and Regeneration Protocol.- 6 Summary and Conclusion.- 7 Culture Media and Solutions.- References.- II.4 Transformation in Chickpea (Cicer arietinum L.).- 1 Introduction.- 2 Agrobacterium Chickpea Interaction.- 3 Analysis of Transformed Tissues.- 4 Summary and Conclusions.- References.- II.5 Transformation in Citrus.- 1 Introduction.- 2 Review of Previous Research on Citrus Transformation.- 3 Transformation Parameters.- 4 Evaluation of Transformation.- 5 Conclusions and Prospects.- 6 Protocol.- References.- II.6 Transformation in Muskmelon (Cucumis melo L.).- 1 Introduction.- 2 A. tumefaciens-Mediated Transformation and Regeneration of Muskmelon Plants.- 3 Summary and Conclusions.- References.- 11.7 Transformation in Cucumber (Cucumis sativus L.).- 1 Introduction.- 2 Materials and Methods.- 3 Experimental Results.- 4 Preliminary Field Testing of Plants Which Express the CMV-C Coat Protein Gene.- 5 Second Field Testing of Transgenic Cucumber Plants That Express the CMV Coat Protein Gene.- 6 Conclusion.- References.- II.8 Transformation in Soybean (Glycine max L.).- 1 Introduction.- 2 Delivery of DNA into Soybean Cells.- 3 Regeneration of Transformed Soybeans.- 4 Conclusions and Future Prospects.- References.- II.9 Transformation of Lettuce (Lactuca sativa L.).- 1 General Account.- 2 Transformation in Lactuca sativa L.- 3 Inoculation of Agrobacterium and Selection of Transformants.- 4 GUS Assay and Kanamycin Resistance in the Self-Pollinated Progeny.- 5 Histochemical Localization of Salicylic Acid and Bacterial Stress-Induced GUS Activity.- 6 Southern Blot Analysis.- 7 Conclusions and Prospects.- 8 Protocol.- References.- II. 10 Transformation of Birds-Foot Trefoil (Lotus corniculatus).- 1 Introduction.- 2 Transformation of Lotus corniculatus.- 3 An Overview of Genetic Transformation of Forage Legumes.- 4 Concluding Remarks.- 5 Protocol.- References.- II.11 Transformation of Tomato (Lycopersicon esculentum Mill.) for Virus Disease Protection.- 1 Introduction.- 2 Review of Transformation Work in Tomato.- 3 Intranuclear Microinjection for Transformation of Tomato Callus Cells.- 4 Transformation of Tomato with Mutagenized Coat Protein (MCP) Gene.- 5 Summary and Conclusion.- References.- II.12 Transformation in Cassava (Manihot esculenta Crantz).- 1 Introduction.- 2 Experiments with a Particle Gun: Transient Expression of the GUS Gene.- 3 Transformation of Leaf Disks with Agrobacterium tumefaciens.- 4 Transformation of Leaf Lobes by Bombardment with an Agrobacterium tumefaciens Suspension.- 5 Transformation of Somatic Embryos with Agrobacterium tumefaciens.- 6 Antibiotics and Selection of Transformed Tissues.- 7 Summary and Conclusions.- References.- II.13 Transformation of Tobacco (Nicotiana clevelandii and N. benthamiana).- 1 Introduction.- 2 Transformation Studies on Nicotiana.- 3 Transformation of Nicotiana clevelandii and N. benthamiana.- 4 Prospects and Conclusions.- References.- II.14 Transformation of Petunia and Corn Plants (Petunia hybrida and Zea mays) Using Agrobacterium tumefaciens and the Shoot Ape.- 1 Introduction.- 2 Methods.- 3 Results.- 4 Discussion.- 5 Assumptions.- 6 Conclusions.- References.- II.15 Transformation in Spruce (Picea Species).- 1 Introduction.- 2 Gene Transfer Systems.- 3 Selection.- 4 Gene Expression.- 5 Regeneration of Transformed White Spruce.- 6 Summary.- References.- 11.16 Transformation of Pea (Pisum sativum L.).- 1 Introduction.- 2 Pea Tissue Culture/Regeneration in Vitro.- 3 Transformation.- 4 Concluding Remarks.- References.- II.17 Transformation of Sugarcane.- 1 Why Genetic Engineering Is Attractive for Sugarcane Variety Improvement.- 2 Sugarcane Tissue Culture.- 3 Gene Transfer Techniques.- 4 Promoters and Marker Genes for Sugarcane Transformation.- 5 Prospects.- References.- II.18 Transformation of Stylosanthes Species.- 1 Introduction.- 2 Tissue and Protoplast Culture of Stylosanthes spp.- 3 Agrobacterium-Mediated Transformation.- 4 Transient Gene Expression in S. humilis Using DNA-Coated Microprojectiles.- 5 Selection of Transformed Tissues.- 6 Reporter Gene Expression in Stably Transformed Tissues.- 7 Genes for Improvement.- 8 Summary.- References.- II.19 Transformation in Grapevine (Vitis spp.).- 1 Introduction.- 2 Genetic Transformation of Grape.- 3 Conclusions and Future Prospects.- References.

Volume

v. 5 : Germany ISBN 9783540574477

Description

In continuation of Volumes 8, 9, 22, and 23, this new volume deals with the regeneration of plants from isolated protoplasts and genetic transformation in various species of Actinidia, Allocasuarina, Anthurium, Antirrhinum, Asparagus, Beta, Brassica, Carica, Casuarina, Cyphomandra, Eucalyptus, Ipomoea, Larix, Limonium, Liriodendron, Malus, Musa, Physcomitrella, Physalis, Picea, Rosa, Tagetes, Triticum, and Ulmus. These studies reflect the far-reaching implications of protoplast technology in genetic engineering of plants. The book contains a wealth of useful information for advanced students, teachers, and researchers in the field of plant tissue culture, molecular biology, genetic engineering, plant breeding, and general biotechnology.

Table of Contents

Section I Regeneration of Plants from Protoplasts.- I.1 Regeneration of Plants from Protoplasts of Asparagus officinalis L..- I.2 Regeneration of Plants from Protoplasts of Beta vulgaris (Sugar Beet).- I.3 Regeneration of Plants from Protoplasts of Brassica juncea (L.) Czern & Coss (Brown Mustard).- I.4 Regeneration of Plants from Protoplasts of Carica Species (Papaya).- I.5 Regeneration of Plants from Protoplasts of Larix Species (Larch).- I.6 Regeneration of Plants from Protoplasts of Statice (Limonium perezii Hubbard).- I.7 Regeneration of Plants from Protoplasts of Malus Xdomestica Borkh. (Apple).- I.8 Regeneration of Plants from Protoplasts of Musa Species (Banana).- I.9 Regeneration of Plants from Protoplasts of Picea Species (Spruce).- I.10 Regeneration of Plants from Protoplasts of Physalis Species.- I.11 Regeneration of Plants from Protoplasts of Rosa Species (Roses).- I.12 Regeneration of Plants from Protoplasts of Triticum aestivum L. (Wheat).- I.13 Regeneration of Plants from Protoplasts of Ulmus Species (Elms).- Section II Genetic Transformation and Transgenic Plants.- II.1 Genetic Transformation in Actinidia deliciosa (Kiwifruit).- II.2 Genetic Transformation in Anthurium.- II.3 Genetic Transformation of Antirrhinummajus L. (Snapdragon).- II4 Genetic Transformation in Carica papaya L. (Papaya).- II.5 Genetic Transformation of Trees in the Casuarinaceae Family.- II.6 Genetic Transformation of Cyphomandra betacea (Tamarillo).- II.7 Genetic Transformation of Eucalyptus.- II8 Genetic Transformation in Ipomoea batatas (L.) Lam (Sweet Potato).- II.9 Genetic Transformation in Larix Species (Larches).- II.10 Genetic Transformation in Liriodendron tulipifera L. (Yellow Poplar).- II.11 Genetic Transformation of the Moss Physcomitrella patens.- II.12 Genetic Transformation in Tagetes Species (Marigolds) for Thiophene Contents.- II.13 Genetic Transformation in Triticum Species (Wheat).- II.14 Genetic Transformation in Ulmus Species (Elms).

Volume

v. 6 : Germany ISBN 9783540589310

Description

Plant protoplasts have proved to be an excellent tool for in vitro manipu- lations, somatic hybridization, DNA uptake and genetic transformation, and for the induction of somaclonal variation. These studies reflect the far- reaching impact of protoplast alterations for agriculture and forest bio- technology. Taking these aspects into consideration, the series of books on Plant Protoplasts and Genetic Engineering provides a survey of the litera- ture, focusing on recent information and the state of the art in protoplast Plant Protoplasts manipulation and genetic transformation. This book, and Genetic Engineering VI, like the previous five volumes published in 1989,1993, and 1994, is unique in its approach. It comprises 27 chapters dealing with the regeneration of plants from protoplasts, and genetic transformation in various species of Arachis, Bupleurum, Capsella, Dendrobium, Dianthus, Diospyros, Fagopyrum, Festuca, Gentiana, Glycyrrhiza, Gossypium, Hemerocallis, Levisticum, Lonicera, Musa, Physallis, Platanus, Prunus, Saposhnikovia, Solanum, Spinacia, Trititrigia, Tulipa, and Vaccinium; including fruits such as apricot, banana, cranberry, pepino, peach, and plum. This book may be of special interest to advanced students, teachers, and research scientists in the field of plant tissue culture, molecular biology, genetic engineering, plant breeding, and general bio- technology. New Delhi, August 1995 Professor Y. P. S. BAJA] Series Editor Contents Section I Regeneration of Plants from Protoplasts 1. 1 Regeneration of Plants from Protop1asts of Arachis Species (Peanut) Z. LI, R. L. JARRET, and J. W. DEMSKI (With 2 Figures) 1 Introduction ...3 2 Isolation of Pro top lasts ...4 3 Culture of Protoplasts ...

Table of Contents

Section I Regeneration of Plants from Protoplasts.- I.1 Regeneration of Plants from Protoplasts of Arachis Species (Peanut).- I.2 Regeneration of Plants from Protoplasts of Bupleurum scor zonerifolium (Red Thorowax) and Saposhnikovia divaricata (Divaricate Saposhnikovia).- I.3 Regeneration of Plants from Protoplasts of Capsella bursa-pastoris (L.) Medic. (Shepherd's Purse).- I.4 Regeneration of Plants from Protoplasts of Dianthus Species (Carnation).- I.5 Regeneration of Plants from Protoplasts of Diospyros kaki L. (Japanese Persimmon).- I.6 Regeneration of Plants from Protoplasts of Gentiana Species (Gentian).- I.7 Regeneration of Plants from Protoplasts of Gossypium Species (Cotton).- I.8 Regeneration of Plants from Protoplasts of Hemerocallis (Daylily).- I.9 Regeneration of Plants from Protoplasts of Levisticum officinale Koch (Lovage).- I.10 Regeneration of Plants from Protoplasts of Lonicera Species (Honeysuckle).- I.11 Regeneration of Plants from Protoplasts of Platanus orientalis (Plane Tree).- I.12 Regeneration of Plants from Protoplasts of Solarium khasianum C.B. Clark and Solanum laciniatum Ait..- I.13 Regeneration of Plants from Protoplasts of Trititrigia (Triticum sect, trititrigia).- Section II Genetic Transformation and Transgenic Plants.- II.1 Genetic Transformation in Arachis hypogaea L. (Peanut).- II.2 Genetic Transformation in Dendrobium (Orchid).- II.3 Genetic Transformation of Dianthus caryophyllus (Carnation).- II.4 Genetic Transformation in Fagopyrum esculentum (Buckwheat).- II.5 Genetic Transformation in Festuca arundinacea Schreb. (Tall Fescue) and Festuca pratensis Huds. (Meadow Fescue).- II.6 Genetic Transformation in Glycyrrhiza uralensis Fisch, et D.C. (Licorice).- II.7 Genetic Transformation in Musa Species (Banana).- II.8 Genetic Transformation in Physalis Species (Tomatillo).- II.9 Genetic Transformation in Prunus armeniaca L. (Apricot).- II. 10 Genetic Transformation in Prunus persica (Peach) and Prunus domestica (Plum).- II. 11 Genetic Transformation of Solanum muricatum (Pepino).- II. 12 Genetic Transformation in Spinacia oleracea L. (Spinach).- II. 13 Genetic Transformation in Tulipa Species (Tulips).- II. 14 Genetic Transformation in Vaccinium macrocarpon Ait. (Cranberry).- References.

Volume

v. 7 ISBN 9783540608769

Description

Plant protoplasts have proved to be an excellent tool for in vitro manipulation, somatic hybridization, DNA uptake and genetic trans- formation, and for the induction of somac1onal variation. These studies reflect the far-reaching impact of protoplast research in agriculture and forest biotechnology. Taking these aspects into consideration, the series of books on Plant Protoplasts and Genetic Engineering provides a survey of the literature, focusing on recent information and the state of the art in protoplast manipulation and genetic transformation. This book, Plant Protoplasts and Genetic Engineering VII, like the previous six volumes published in 1989, 1993, 1994, and 1995, is unique in its approach. It comprises 27 chapters dealing with the regeneration of plants from protoplasts, and genetic transformation in various species of Agrostis, Allium, Anthriscus, Asparagus, Avena, Boehmeria, Carthamus, Coffea, Funaria, Geranium, Ginkgo, Gladiolus, Helianthus, Hordeum, Lilium, Lithospermum, Mentha, Panax, Papaver, Passiflora, Petunia, Physcomi- trella, Pinus, Poa, Populus, Rubus, Saintpaulia, and Swertia. This book may be of special interest to advanced students, teachers, and research scientists in the field of plant tissue culture, molecular biology, genetic engineering, plant breeding, and general biotechnology. New Delhi, June 1996 Professor y. P. S. BAJAJ Series Editor Contents Section I Regeneration of Plants from Protoplasts 1. 1 Regeneration of Plantlets from Protoplasts of Allium cepa (Onion) E. E. HANSEN, J. F. HUBSTENBERGER, and G. C. PHILLIPS (With 3 Figures) 1 Introduction ...3 2 Protoplast Isolation ...4 3 Protoplast Culture ...8 4 Regeneration of Plantlets ...9 5 Summary...

Table of Contents

Section I Regeneration of Plants from Protoplasts.- I. 1 Regeneration of Plantlets from Protoplasts of Allium cepa (Onion).- I. 2 Regeneration of Plants from Protoplasts of Anthriscus sylvestris (L.) Hoffm. (Woodland Beakchervil).- I. 3 Regeneration of Plants from Protoplasts of Coffea spp. (Coffee).- I. 4 Direct Embryogenesis in Protoplasts of Ginkgo biloba (Maidenhair Tree).- I. 5 Regeneration of Plants from Protoplasts of Helianthus annuus L. (Sunflower).- I. 6 Regeneration of Plants from Protoplasts of Hordeum vulgare (Barley).- I. 7 Regeneration of Plants from Protoplasts of Lilium x formolongi.- I. 8 Regeneration of Plants from Protoplasts of Mentha piperita L. (Peppermint).- I. 9 Regeneration of Plants from Protoplasts of Mosses (Funaria hygrometrica and Physcomitrella patens).- I. 10 Regeneration of Plants from Protoplasts of Passiflora Species (Passion Fruit).- I. 11 Regeneration of Plants from Protoplasts of Poa pratensis L. (Kentucky Blue Grass).- I. 12 Regeneration of Plants from Protoplasts of Populus Species (Poplars).- I. 13 Regeneration of Plants from Protoplasts of Saintpaulia ionantha H. Wendl. (African Violet).- Section II Genetic Transformation and Transgenic Plants.- II. 1 Genetic Transformation in Agrostis palustris Huds. (Creeping Bentgrass).- II. 2 Genetic Transformation in Asparagus officinalis L..- II. 3 Genetic Transformation in Avena sativa L. (Oat).- II. 4 Genetic Transformation in Boehmeria nivea Gaud. (Ramie Fiber).- II. 5 Genetic Transformation of Carthamus tinctorius L. (Safflower).- II. 6 Genetic Transformation of Geraniums.- II. 7 Genetic Transformation of Gladiolus.- II. 8 Genetic Transformation of Lithospermum erythrorhizon for Increased Production of Shikonin.- II. 9 Genetic Transformation in Papaver somniferum L. (Opium Poppy) for Enhanced Production of Morphinan.- II. 10 Genetic Transformation of Panax ginseng (C. A. MEYER) for Increased Production of Ginsenosides.- II. 11 Genetic Transformation in Petunia.- II. 12 Genetic Transformation in Pinus elliottii Engelm. (Slash Pine).- II. 13 Genetic Transformation in Raspberries and Blackberries (Rubus Species).- II. 14 Genetic Transformation in Swertia japonica.

by "Nielsen BookData"

Details

• NCID: BA07215938
• ISBN:
  • 3540191941
  • 0387191941
  • 3540507892
  • 0387507892
  • 354056313X
  • 038756313X
  • 3540563849
  • 0387563849
  • 3540574476
  • 0387574476
  • 3540589317
  • 0387589317
  • 3540608761
• LCCN: 88031197
• Country Code: gw
• Title Language Code: eng
• Text Language Code: eng
• Place of Publication: Berlin ; Tokyo
• Pages/Volumes: v.
• Size: 25 cm
• Classification:
  • NDC8 : 471.1
  • LCC : QK725
  • DC19 : 581.87/3
• Subject Headings:
  • LCSH : Plant protoplasts
  • LCSH : Plant genetic engineering
• Parent Bibliography ID:
  • BA00489232