Manipulating secondary metabolism in culture

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書誌事項

Manipulating secondary metabolism in culture

edited by Richard J. Robins & Michael J.C. Rhodes

Cambridge University Press, 1988

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注記

"Presentations made to the Second Meeting of the UK Section of the International Association for Plant Tissue Culture ... held at the AFRC Institute of Federal Research, Norwich, on the 16th and 17th September 1987"--Pref

Includes bibliographies and indexes

内容説明・目次

内容説明

This volume surveys the most recent and exciting advances in the practical utilisation of biotechnology to produce useful medicinal, colouring and flavour compounds from plant cell cultures. The volume shows how plant cells can be induced to form these so-called 'secondary' products in defined biotechnological conditions and describes the way this is activated in several species. It also covers recent developments in this field towards the selection of cells with improved characteristics for making these products, in particular the new approach of using transformation to provide organised cultures. The volume provides an important account of the practicalities and techniques involved in the exploitation of commercially important secondary plant products. The volume will be particularly useful for biotechnologists, plant biochemists and research staff developing new chemicals and drugs.

目次

  • Preface
  • Acknowledgements
  • Plenary lecture
  • 1. The genetic manipulation of antibiotic production in Streptomyces
  • Part I. Genetic and Biochemical Variation in Culture: 2. Variation in the secondary metabolism of cultured plant cells
  • 3. DNA methylation as a control phenomenon in plant cell culture
  • 4. Aspects of sesquiterpene phytoalexin formation in elicited cell suspension cultures of Nicotiana tabacum: accumulation, metabolism and enzymology
  • 5. The use of metal ions to induce the formation of secondary products in plant tissue culture
  • 6. Elicitation of cell cultures
  • 7. The effects of fungal elicitation on secondary metabolism in cell cultures of Capsicum frutescens
  • 8. Perturbation of alkaloid biosynthesis in transformed root cultures of solanaceous plants by metabolic precursors and their analogues
  • 9. Rapid assay of tryptophan decarboxylase from plant cell culture
  • Part II. Improvement by Screening and Selection: 10. Strain improvement by screening and selection techniques
  • 11. Screening of calli and compact globular structures of Cinchona ledgeriana for production of quinoline alkaloids
  • 12. Exploiting variation in tropane alkaloid production by solanaceous plants
  • 13. Changes in growth and nutritional requirements of cultured Nicotiana tabacum cv. Wisconsin-38 cells with increasing dependency upon photosynthesis for the provision of organic constituents
  • Part III. Improvement by Genetic Manipulation: 14. Plant genetic transformation methods
  • 15. Prospects for the genetic manipulation of complex metabolic pathways
  • 16. Chromosome stability in transformed hairy root cultures and the properties of variant lines of Nicotiana rustica hairy roots
  • 17. Foreign gene insertion into transformed roots with binary vectors and Agrobacterium rhizogenes - potential for genetic manipulation of secondary metabolism
  • Part IV. Biotechnology for Food Flavours and Colours: 18. The value of plant biotechnology to the flavour industry
  • 19. Fatty acid synthesis in Theobroma cacao beans and suspension cultures
  • 20. Propenylcysteine sulphoxide biosynthesis in callus cultures of Allium cepa (L)
  • 21. Essential oil accumulation in shoot proliferation cultures of Pelargonium spp.
  • 22. The accumulation of flavour compounds by cultures of Zingiber officinale
  • Part V. Biotechnology for Producing Fine Chemicals: 23. Medical products through plant biotechnology
  • 24. Effects of elicitors on the production of secondary metabolites in plant cell cultures of Cinchona, Rubia, Morinda and Tabernaemontana
  • 25. Selection of thiophene-producing calli of Tagetes patula in relation to the production of thiophenes in liquid cultures
  • 26. Thiophene production from 'hairy roots' of Tagetes
  • 27. Transformated root cultures - fermentation aspects
  • Part VI. Techniques: 28. Flow cytometry and cell sorting as tools for culture improvement
  • 29. Red beet batch culture: demonstration of a bubble-free Taylor-Couette bioreactor
  • 30. Co-immobilization of urease and microbial cells
  • 31. The axenic isolation of plant protoplasts using Percoll
  • 32. Assay of gene expression by immunodetection
  • 33. Electropermeabilization and ultrasonic techniques for harvesting secondary metabolites from plant cells in vitro
  • 34. Rapid identification of secondary products in plant cell cultures by thermospray liquid chromatography/mass spectrometry
  • Species index
  • Subject index.

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