PCR technology : principles and applications for DNA amplification
著者
書誌事項
PCR technology : principles and applications for DNA amplification
（Breakthroughs in molecular biology）
Macmillan Publishers , Stockton Press, 1989
- : uk
- : us
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内容説明・目次
内容説明
This is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years.
目次
- Part 1 Introduction to the PCR: history of PCR development
- description of the PCR method - basic principles and properties of the PCR
- thermostable DNA polymerases
- automation
- sample preparation. Part 2 Research applications - brief overview: direct sequencing using PCR
- preparation of specific DNA fragments using PCR
- detection of gene expression
- genetic mapping using PCR amplification of individual gametes. Part 3 Diagnostic applications - brief overview: detection of pathogens
- diagnosis of monogenic genetic diseases
- HLA DNA typing and disease susceptibility
- forensic applications
- detection of activated oncogenes
- future developments and applications.
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