Immunochemical techniques for the identification and estimation of macromolecules
著者
書誌事項
Immunochemical techniques for the identification and estimation of macromolecules
（Laboratory techniques in biochemistry and molecular biology, v. 1,
Elsevier, 1988
3rd rev. ed.
- : pocket edition
- : library edition
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内容説明・目次
内容説明
Rapid expansion in the field of immunochemistry has necessitated the publication of a new edition to succeed the indispensable present manual. As its predecessor, this revised edition presents you with the data and practical advice you need on the application of the rapidly expanding mass of data on immunochemistry. More specifically, the book examines the possibilities of employing immunological microtechniques in biochemical and histochemical analyses. The reader will find up-to-date information on the practical application of immunochemical analysis for laboratory use. Immunochemical Techniques especially stresses the possible errors and misinterpretations which may occur. Revised and extended chapters include: lectins, HLA-antigens, and crossed-electrophoresis. New chapters deal with: the complement system, enzyme immunoassay systems, immuno-fluorescence methods, immunoadsorbent techniques, and recent developments in methodology. Conveniently available in hardcover and paperback, this volume is unsurpassed both as a practical reference work, and as a laboratory manual.
目次
(Chapter headings) Preface. Chapters: 1. Introduction 2. Immunodiffusion in gels 3. Antisera 4. Techniques of immunodiffusion 5. Techniques of immunoelectrophoresis 6. Visualization and interpretation of precipitates in gels 7. Immunoadsorbent techniques 8. Radio-immunochemical techniques 9. Enzymo- and metallo-immunoassays 10. Difficulties and artifacts arising in gel diffusion and in immunoelectrophosesis 11. Immunochemical reactions in free buffer systems 12. Immunofluorescence techniques 13. Complement fixation assays 14. Application of lymphocytes for tracing antigens 15. Miscellaneous topics in immunochemistry 16. Appendices References. Subject index.
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