Yeast genetic engineering
著者
書誌事項
Yeast genetic engineering
（Biotechnology series, 13）
Butterworths, c1989
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注記
Includes bibliographies and index
内容説明・目次
内容説明
An illustration of the impressive development of yeast gene expression systems accomplished in recent years which demonstrates the power of these systems for the production of proteins in quantities useful as therapeutics, industrial enzymes and research agents. It provides a foundation for the comprehensive study of the molecular biology of yeast and describes and reviews many basic aspects of yeast biochemistry and cell biology at the DNA, RNA and protein levels. The book covers, at the molecular genetic level, processes involved in the natural metabolism and propagation of yeasts. In addition, it superimposes the modern methods of molecular biology and biotechnology on this background of classical information. The first recombinant subunit vaccine against hepititis B, for example, is manufactured from genetically engineered yeast.
目次
- Molecular genetics of galactose metabolism
- the lactose-galactose regulon of "kluyveromyoes lactis"
- phospate regulation in yeast
- fission yeast
- the role of yeast mRNA sequences and structures in translation
- regulated transcription systems for the production of proteins in yeast - regulation by carbon source
- targeting of proteins for degradation
- the yeast retrotransposon Ty - molecular genetics and exploitation
- propagation and expression of genes in yeast using 2-micron circle vectors
- heterologous gene expression in yeast
- yeast mutants with increased secretion efficiency
- glycosylation of heterologous proteins in "saccharomyoes cerevisiae"
- cellulcolytic yeast strains and their applications
- secretion of heterologous protein directed by the yeast factor leader
- use of cDNA expression-cloning vector and secretion vector for mammalian lymphokine gene expression in "Sacchoromyoes cerevisiae"
- synthesis and processing of thaumatin in yeast
- recombinant subunit vaccines from yeast.
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