The Use of HPLC in receptor biochemistry

Bibliographic Information

The Use of HPLC in receptor biochemistry

editor, Anthony R. Kerlavage

(Receptor biochemistry and methodology, v. 14)

A.R. Liss, c1989

  • hard

Available at  / 7 libraries

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Note

Includes bibliographies and index

Description and Table of Contents

Description

This text offers a concise background in HPLC methodology and includes illustrative examples of some of the key advances made in the receptor field through application of this chromatographic technique. A brief historical overview puts into perspective the specific state-of-the-art of HPLC laboratory applications that are contained in the book's chapters. Technical contributions discuss how best to select the HPLC mode for a particular biochemical problem, including the most effective use of a particularly sensitive technique, microbore HPLC for synthesis, purification and characterization of synthetic peptides. The text will benefit biochemists and molecular biologists.

Table of Contents

  • The isolation of endogenous peptides, with an emphasis on their biologic activity
  • the application of HPLC to receptor mapping (illustrated with case studies of the structural features of receptors discovered with this technique)
  • the use of immunoaffinity chromatography to recover biologically active proteins
  • the application of a wide range of HPLC modes to the study of steroid hormone receptors
  • a highly detailed strategy for isolating small amounts of proteins and peptides for microsequence analysis.

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