Guide to protein purification
Author(s)
Bibliographic Information
Guide to protein purification
(Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan, v. 182)
Academic press, c1990
- : hardcover
- : comb bound
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Library & Science Information Center, Osaka Prefecture University
: hardcoverNDC6:493.8||C||18210091666626
-
Okayama University Institute of Plant Science and Resources Branch Library
: hardcover157||575S205000204172*
-
Okayama University Library, Shikata Branch Library鹿田図
: HardcoverS104100000081*,
: comb boundS104100000088*
Note
Includes bibliographies and indexes
Description and Table of Contents
- Volume
-
: hardcover ISBN 9780121820831
Description
Guide to Protein Purification, designed to serve the needs of the student, experienced researcher and newcomer to the field, is a comprehensive manual that provides all the up-to-date procedures necessary for purifying, characterizing, and handling proteins and enzymes in one source.
Table of Contents
(Section Headings): Developing Purification Procedures. General Methods for Handling Proteins and Enzymes. Optimization of Starting Materials. Preparation of Extracts and Subcellular Fractionation. Solubilization Procedures. Purification Procedures: Bulk Methods. Purification Procedures: Chromatographic Methods. Purification Procedures: Electrophoretic Methods. Specialized Purification Procedures. Characterization of Purified Proteins. Immunological Procedures. Additional Techniques. Concluding Remarks. Appendix: Personal Perspectives on Enzyme Purification. Author Index. Subject Index.
- Volume
-
: comb bound ISBN 9780122135859
Description
"The Guide to Protein Purification" is a self-contained volume covering all the important procedures for purifying proteins, as well as other more specialized techniques. However, to stay within the confines of a single volume, some details are dealt with by reference to other works, but these have been kept to a minimum. It is hoped that this volume will satisfy the needs of both the novice in protein purification and the more experienced researcher. It contains procedures newly written for this volume; and provides extensive practical information. It clearly describes rationale and strategies for protein and enzyme purification. It offers personal perspectives on enzyme purification by eminent researchers.
Table of Contents
Developing Purification Procedures. General Methods for Handling Proteins and Enzymes. Optimization of Starting Materials. Preparation of Extracts and Subcellular Fractionation. Solubilization Procedures. Purification Procedures: Bulk Methods. Purification Procedures: Chromatographic Methods. Purification Procedures: Electrophoretic Methods. Specialized Purification Procedures. Characterization of Purified Proteins. Immunological Procedures. Additional Techniques. Concluding Remarks. Personal Perspectives on Enzyme Purification. Index.
by "Nielsen BookData"