Guide to protein purification

Author(s)

• Deutscher, Murray P.

Bibliographic Information

Guide to protein purification

edited by Murray P. Deutscher

（Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan, v. 182）

Academic press, c1990

• : hardcover
• : comb bound

Note

Includes bibliographies and indexes

Description and Table of Contents

Volume

: hardcover ISBN 9780121820831

Description

Guide to Protein Purification, designed to serve the needs of the student, experienced researcher and newcomer to the field, is a comprehensive manual that provides all the up-to-date procedures necessary for purifying, characterizing, and handling proteins and enzymes in one source.

Table of Contents

(Section Headings): Developing Purification Procedures. General Methods for Handling Proteins and Enzymes. Optimization of Starting Materials. Preparation of Extracts and Subcellular Fractionation. Solubilization Procedures. Purification Procedures: Bulk Methods. Purification Procedures: Chromatographic Methods. Purification Procedures: Electrophoretic Methods. Specialized Purification Procedures. Characterization of Purified Proteins. Immunological Procedures. Additional Techniques. Concluding Remarks. Appendix: Personal Perspectives on Enzyme Purification. Author Index. Subject Index.

Volume

: comb bound ISBN 9780122135859

Description

"The Guide to Protein Purification" is a self-contained volume covering all the important procedures for purifying proteins, as well as other more specialized techniques. However, to stay within the confines of a single volume, some details are dealt with by reference to other works, but these have been kept to a minimum. It is hoped that this volume will satisfy the needs of both the novice in protein purification and the more experienced researcher. It contains procedures newly written for this volume; and provides extensive practical information. It clearly describes rationale and strategies for protein and enzyme purification. It offers personal perspectives on enzyme purification by eminent researchers.

Table of Contents

Developing Purification Procedures. General Methods for Handling Proteins and Enzymes. Optimization of Starting Materials. Preparation of Extracts and Subcellular Fractionation. Solubilization Procedures. Purification Procedures: Bulk Methods. Purification Procedures: Chromatographic Methods. Purification Procedures: Electrophoretic Methods. Specialized Purification Procedures. Characterization of Purified Proteins. Immunological Procedures. Additional Techniques. Concluding Remarks. Personal Perspectives on Enzyme Purification. Index.

by "Nielsen BookData"

Details

• NCID: BA10086389
• ISBN:
  • 0121820831
  • 0122135857
• LCCN: 54009110
• Country Code: us
• Title Language Code: eng
• Text Language Code: eng
• Place of Publication: San Diego ; Tokyo
• Pages/Volumes: xxix, 894 p.
• Size: 25 cm
• Classification:
  • NDC8 : 464.5
  • NLM : W1
• Subject Headings:
  • MESH : Proteins - isolation & purification
• Parent Bibliography ID:
  • BA00197543