Protein engineering : approaches to the manipulation of protein folding

書誌事項

Protein engineering : approaches to the manipulation of protein folding

edited by Saran A. Narang

(Biotechnology series, 14)

Butterworths, c1990

大学図書館所蔵 件 / 14

この図書・雑誌をさがす

注記

Includes bibliographical references and index

内容説明・目次

内容説明

This book addresses the theoretical implication of the protein problem and outlines new genetic tools to synthesize gene coding for novel proteins, site-directed mutagenesis, gene transposition, and phenotype selection procedure. In addition, it describes various physiochemical tools such as x-ray crystallography and protein spectroscopy to study protein structure. The knowledge gained using this kind of integrated approach will eventually allow scientists to design synthetic enzymes, hormones and structural proteins, thus opening up the new area of protein folding. It is the intent of this book to draw the attention of a new generation of scientists to the folding problem challenge.

目次

  • Theories and simulation of protein folding
  • experimental approaches to protein folding
  • sito-directed mutagenesis and its applications to protein folding
  • the elucidation and engineering of sites that affect the activity of an enzyme of unknown structure
  • structural and functional features of the HIV envelope and considerations for Vaccine Development
  • crystallographic determination of protein structure
  • the conformation of proteins and peptides in a membrance environment - an infrared spectroscopic approach
  • applications of laser-based fluoresence techniques to study protein structure and dynamics
  • protein structure determination by nuclear magnetic resonance spectrosopy
  • synthetic transposons
  • redesigning genes.

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