Current research in protein chemistry : techniques, structure, and function
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書誌事項
Current research in protein chemistry : techniques, structure, and function
Academic Press, c1990
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注記
Papers from the Third Annual Meeting of the Protein Society held at the University of Washington in Seattle, Jul. 29-Aug. 2, 1989
"Published under the auspices of the Protein Society."
Includes bibliographical references and index (p. 589-606)
内容説明・目次
内容説明
Current Research in Protein Chemistry: Techniques, Structure, and Function focuses on the techniques and methods used for determining the structure and function of proteins. Topics covered range from protein folding and stability to catalysis by chimeric proteins, amino acid and peptide analysis, applications of mass spectrometry to peptide and protein analysis, and protein sequencing. This book is divided into six sections encompassing 55 chapters. The first chapter describes a novel method for protein hydrolysis by means of microwave irradiation that uses Teflon-Pyrex tubes. This is followed by a discussion of the application of high performance capillary electrophoresis to the analysis of amino acids. The sections that follow focus on mass spectrometric methods, protein sequencing, and capillary electrophoresis as well as protein stability, chimeric proteins and enzyme modifications, and protein structure prediction. The crystal structure of human interleukin-1alpha, the acid-denatured states of proteins, solubility of recombinant proteins expressed in Escherichia coli, and catalysis by chimeric proteins are considered. The reader is also introduced to peptide mapping and internal sequencing of proteins from acrylamide gels, new approaches to covalent sequence analysis, alkaline denaturation of hemoglobin, and measurements of disulfide bond stabilities in protein folding intermediates. Students and researchers interested in protein chemistry will find this book extremely helpful.
目次
- Foreword Preface Dedication to Emil T. Kaiser and Irving S. Sigal AcknowledgmentsSection I Techniques: Amino Acid Analysis
- Peptides and Proteins 1. A Rapid and Novel Means of Protein Hydrolysis by Microwave Irradiation Using Teflon-Pyrex Tubes 2. Analysis of Amino Acids by High Performance Capillary Electrophoresis 3. An Evaluation of Microwave Heating for the Vapor Phase Hydrolysis of Proteins: I. Comparison to Vaper Phase Hydrolysis for 24 Hours 4. Performance Evaluation: Automatic Hydrolysis and PTC Amino Acid Analysis 5. A Collaborative Amino Acid Analysis Study from the Association of Biomolecular Resource Facilities 6. Recent Developments in Solid Phase Peptide Synthesis Using the 9-Fluorenylmethyloxycarbonyl (FMOC) Protecting Group Strategy 7. Relationships of Paramecium and Endocrine Secretory Proteins 8. Quantitative Electrotransfer of Proteins from Polyacrylamide Gels onto PVDF Membranes 9. Selective Isolation of Disulfide-Containing Peptides from Trypsin Digests Using Strong Cation Exchange HPLCSection II Techniques: Mass Spectrometric Methods
- Protein Sequencing
- Capillary Electrophoresis 10. Analysis of Peptides and Proteins by Tandem Quadrupole Mass Spectrometry 11. Protein and Oligopeptide Sequence Analysis on the TSQ-70 Triple Quadrupole Mass Spectrometer 12. Sequence Analysis of Peptides by Plasma Desorption Mass Spectrometry 13. Study of the Primary Structure of Recombinant Proteins with a Benchtop Gas Chromatograph-Mass Spectrometer-Computer Data Syste 14. Synthesis and Mass Spectrometry of Phosphotyrosine-Containing Peptides 15. Design, Characterization, and Results of ABRF-89SEQ: A Test Sample for Evaluating Protein Sequencer Performance in Protein Microchemistry Core Facilities 16. Extended N-Terminal Protein Sequencing of Clostridium botulinum Neurotoxin Type A Fragments: Continuous Sequence to Seventy Residues Both from Glass-Fiber Filter Using the Gas Phase and Polyvinylidene Difluoride Using the Pulsed Liquid Phase Sequence 17. Peptide Mapping and Internal Sequencing of Proteins from Acrylamide Gels 18. New Approaches to Covalent Sequence Analysis 19. Analysis of Brain Constituents by Capillary Electrophoresis 20. Capillary Electrophoresis Analysis of Species Variations in the Tryptic Maps of Cytochrome C 21. Ultrafast Protein Analysis: A Powerful Technique for Recombinant Protein Process Monitoring 22. Separation and Characterization of Proteins in the Range of 1 to 200 kDa with HPEC (TM) 23. Protein Detection during Poly aery lamide Gel Electrophoresis Using High Resolution Dynamic ImagingSection III Protein Folding 24. Design, Computer Derived Structure and Biological Activity of Three Bicyclic Gonadotropin Releasing Hormone (GnRH) Antagonists 25. The H-Helix of Myoglobin as a Potential Independent Protein Folding Domain 26. Probing the Role of Position 49 in the Folding of the Alpha Subunit of Tryptophan Synthase from Escherichia coli 27. Tests of Isolated ss-Sheet Formation in a Cyclic Peptide from BPTI 28. Spectroscopic and Chromatographic Studies of Native and Denatured States of T4 Lysozymes 29. Studies of the Alkaline Denaturation of Hemoglobin in an NMR Optical Stopped-Flow Apparatus 30. Conformational Studies of Peptides Corresponding to the LDL Receptor Cytoplasmic Tail and Transmembrane Domain 31. Biochemical and Biophysical Comparison of Bacterial DnaK and Mammalian hsc73, Two Members of an Ancient Stress Protein FamilySection IV Protein Structure/Stability 32. The Crystal Structure of Human Interleukin-1a 33. Crystal Structure of a Protein-Toxin Solved from a Predicted Model 34. Segmental Accommodation: A Novel Conformational Change Induced upon Ligand Binding by Thymidylate Synthase 35. Subunit Interactions and the Function of Tumor Necrosis Factor 36. Structure Prediction Methods for Membrane Proteins: Comparison with the X-Ray Structure of the R. viridis Photosynthetic Reaction Centre 37. The Sequence Attributes Method for Determining Correlations Between Amino Acid Sequence and Protein Secondary Structure 38. Acid-Denatured States of Proteins 39. Estimation of Protein Stability: Comparison of Calorimetric and Denaturant Studies 40. Engineering of Multiple Disulfide Bonds Dramatically Stabilizes T4 Lysozyme 41. E. coli Thioredoxin Stability is Greatly Enhanced by Substitution of Aspartic Acid 26 by Alanine 42. Measurements of Disulfide Bond Stabilities in Protein Folding Intermediates 43. Solubility of Recombinant Proteins Expressed in Escherichia coliSection V Chimeric Proteins and Enzyme Modifications 44. Multiple Determinants for Substrate Specificities in Cytochrome P450 Isozymes 45. Catalysis by Chimeric Proteins: Conversion of a ss-Lactamase to a D,D-Carboxypeptidase 46. Molecular Mapping of Immunogenic Determinants of Human CD4 Using Chimeric Interspecies Molecules and Anti-CD4 Antibodies 47. Ecballium elaterium Trypsin Inhibitor II (EETI II) as a Vehicle for New Functions 48. Comparison of the Dimerization, Stability and Inhibitor Binding Between HIV-1 Protease Wild Type and Active Site Mutant 49. Structure/Function Analysis of IL-2 Toxin: Fragment B Requirements for the Translocation of Fragment A into the Cytosol of Target Cells 50. Alteration of the Site of Adenylylation of Glutamine Synthetase by Site-Directed MutagenesisSection VI Structure Prediction Workshop 51. Three-Dimensional Protein Structure Prediction Workshop: Overview and Summary 52. Protein Structure Prediction 53. Knowledge-Based Protein Modelling: Human Plasma Kallikrein and Human Neutrophil Defensin 54. Application of a Knowledge-Based Strategy to the Prediction of Protein Tertiary Structure 55. Structure Determination of Haloalkane DehalogenaseIndex
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