In situ hybridization : principles and practice
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Bibliographic Information
In situ hybridization : principles and practice
(Oxford science publications)
Oxford University Press, 1990
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Note
p. ix-x missing (1991 reprint)
Description and Table of Contents
Description
"In situ" hybridization is a technique of molecular biology that is used to localize the genetic machinery (genes) involved in protein production, by the use of complimentary strands of genetic material (oligonucleotides of DNA or RNA). It is important in the study of the mechanisms underlying cellular function and disease. This book covers all aspects of "in situ" hybridization, from the basic principles of molecular biology, through the various methodological advances, to its most up-to-date applications in pathology.
Table of Contents
- Basic background of molecular biology, V.T-W.Chan et al
- principles of "in situ" hybridization, H.Hofler
- radioactive labels - autoradiography and choice of emulsions for "in situ" hybridization, M.A.W.Brady and M.A.Finlan
- non-radioactive probes - preparation, characterization, and detection, V.T-W.Chan and J.O'D.McGee
- oligonucleotide probes for "in situ" hybridization, R.Lathe
- principles and applications of complementary RNA probes, S.J.Gibson and J.M.Polak
- quantification of radioactive "in situ" hybridization signals, A.P.Davenport and D.J.Nunez
- "in situ" hybridization and the study of development, D.G.Wilkinson
- "in situ" hybridization in virology, C.G.Teo
- chromosomal assignment of genes, B.Bhatt and J.O'D.McGee
- interphase cytogenetics of solid tumours, A.H.N.Hopman et al
- single and simultaneous nucleic acid detection in archival human biopsies - application of non-isotopic "in situ' hybridization and the polymerase chain reaction to the analysis of human and viral genes, C.S.Herrington et al
- photomicrography, S.Bradbury.
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