Radioisotopes in biology : a practical approach
著者
書誌事項
Radioisotopes in biology : a practical approach
（The practical approach series）
IRL Press at Oxford University Press, c1990
- : pbk
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内容説明・目次
- 巻冊次
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ISBN 9780199630806
内容説明
Radioisotopes have a wide variety of uses in biological investigations. This book presents practical protocols for application in several types of investigation, including in vivo and in vitro labelling, autoradiography and radioimmunoassay. Also included are guidelines to isotope and equipment choice, safety precautions, radiation protection advice and summaries of legislation covering radioactive material in both the USA and the UK. The use of radioactive material in biological research continues to expand, and this book provides practical protocols on how to choose, handle and make best use of it.
目次
- D. Prime & B. Frith: Radioisotope use
- Professor Simmonet: Detection: The scintillation counter
- R. A. Laskey: Detection: Autoradiography
- R. Griffiths, & S. A. Boffey: In vivo labelling
- M. Cunningham: In vitro labelling
- R. Cumming & R. Fallon: Sub-cellular localisation of biological molecules
- R. Thorpe, A. Bristow, & A. Gearing: Radioimmunoassay
- D. Prime & B. Frith: Legislation UK/EEC
- N. S. Nelson: Legislation USA.
- 巻冊次
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: pbk ISBN 9780199630813
内容説明
The value of radioisotopes to biological research is undisputed. Without their availability and use we would be ignorant of a vast range of physiological and biochemical processes, including intermediatary metabolism, neurochemistry, hormone action and protein synthesis. The range and detail of applications is vast and beyond the scope of any book. However, there are general principles and techniques common to all or most radiotracer experiments: in the first place,
the decisions to use then chose a radioisotope; secondly, the establishment of a suitable working environment conducive to safe laboratory practice and the compliance with relevant legislation; thirdly, the principles of safe and efficient experimental design, and finally the radiation detection and
data analysis. These features, essential to any radioisotope experiment, are fully explored in this book. explores these
目次
- D. Prime & B. Frith: Radioisotope use
- Professor Simmonet: Detection: the scintillation counter
- R. A. Laskey: Detection: autoradiography
- R. Griffiths, & S. A. Boffey: In vivo labelling
- M. Cunningham: In vitro labelling
- R. Cumming & R. Fallon: Sub-cellular localisation of biological molecules
- R. Thorpe, A. Bristow, & A. Gearing: Radioimmunoassay
- D. Prime & B. Frith: Legislation UK/EEC
- N. S. Nelson: Legislation USA
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