Gene cloning : an introduction
著者
書誌事項
Gene cloning : an introduction
Chapman and Hall, 1990
2nd ed
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This book is an introduction to the world of gene cloning assuming almost no background knowledge. In the second edition the author has responded to quickening interest in biotechnology by contributing separate chapters on gene cloning applications to medicine and agriculture. New topics in medicine are diagnosis (via DNA probes) and research into genetic diseases, the use of gene cloning in research into cancer and AIDS, and genetic fingerprinting. The agriculture chapter will survey current research into crop improvements. The author has updated the book (three years is a long time in gene research), and added new material whenever appropriate. Among the new topics are some new vectors; the polymerase chain reaction; genomic and RNA sequencing; and analysis of DNA-protein interactions.
目次
- The basic principles of gene cloning
- vehicles - plasmids and bacteriophages
- purification of DNA from living cells
- manipulation of purified DNA
- introduction of DNA into living cells
- cloning vectors for E.coli
- cloning vectors for organisms other than E.coli
- the applications of cloning in gene analysis
- studying gene location and structure
- studying gene expression
- gene cloning in research and biotechnology
- gene cloning in medicine.
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