Preparations of vertebrate central nervous system in vitro
著者
書誌事項
Preparations of vertebrate central nervous system in vitro
（IBRO handbook series : methods in the neurosciences, v. 13）
Wiley, 1990
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注記
"A Wiley-Interscience publication"
Includes bibliographies and index
内容説明・目次
内容説明
Scientists in general acknowledge that information on new methods should be spread throughout the scientific community quickly because awareness of techniques may facilitiate the formulation of new and interesting hypotheses. The purpose of this book is to present to a broad spectrum of investigators some "in vitro" techniques that can be used to study the vertebrate central nervous system. Earlier "in vitro" preparation dominated in the laboratories. Then, with the advent of the brain slice, people realized that tissue from the CNS is not as vulnerable to "non-physiological" conditions as was thought previously. This opened up a new ara in neuroscience and today a large number of "in vitro" preparations are available. The brain slice has been described in many publications. It is essentially a section of the central nervous system in time and space, and this of course puts certain limits on the use of the technique. For instance, it is difficult to study properties of large three-dimensional neural networks. It is equally difficult to study processes lasting more than 18-24 hours.
In this book the authors have sought to point out solutions to these problems as well as to indicate how it is possible to push "in vitro" techniques towards their limits.
目次
- Part 1 Slice preparations: Acutely exposed hippocampal neurons - a preparation for patch clamping neurons from adult hippocampal slices, R.Gray et al
- use of brain slices in the study of serotonergic pacemaker neurons of the brainstem raphe nuclei, G.Aghajanian
- use of slices for quantitative pharmacology, M.A.Simmons
- cultures of brain slices, B.Gahwiler and T.Knopfep. Part 2 En bloc preparations: The lamprey CNS in vitro, an experimentally amenable model for synaptic transmission and intefrative functions, L.Brodin and S.Grillner
- the isolated turtle brain and the physiology of neuronal circuits, J.Hounsgaard and C.Nicholson
- the rabbit retina in vitro, A. Ames and R.H.Masland
- the isolated and perfused mammalian hypothalamus, C.W.Bourque.
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