Flow cytometry : a practical approach

書誌事項

Flow cytometry : a practical approach

edited by M.G. Ormerod

(The practical approach series)

Oxford University Press, c1990

  • : pbk.

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注記

Includes bibliographical references

内容説明・目次

巻冊次

ISBN 9780199630523

内容説明

The flow cytometer is a form of specialized microscope distinguished by a flow chamber which enables particles to be measured. This book details the design of a flow cytometer and presents a series of descriptions of different applications of the instrument. These concentrate on the methods for preparing samples with guidelines for their analysis. The recent explosion in the field of flow cytometry and the rapid increase in the number of machines being installed has resulted in many comparatively inexperienced workers trying to cope with this new technology. This book offers practical guidance in preparing and analysing samples. It will also act as a first point of reference for established workers who wish to add their repertoire of applications.

目次

  • Introduction to the principles of flow cytomery, N.Carter and E.W.Meyer
  • an introduction to fluorescience technology, M.G.Ormerod
  • measurement of cellular subsets using antibodies, N.P.Carter
  • analysis of DNA, M.G.Ormerod et al
  • cell sorting, L.K.Borysiewicz et al
  • chromosome analysis and sorting, B.D.Young
  • intracellular ionized calcium, membrane potential and pH, P.S.Rabinovitch and C.H.June
  • flow cytomery of RNA content, H.J.Tanke
  • nuclear associated antigens, J.V.Watson
  • analysis of radiation and chemical damage in mammilian cells, A.T.M.Vaughan et al
  • measurement of dynamic cellular events, J.V.Watson et al
  • further applications to cell biology, M.G.Ormond.
巻冊次

: pbk. ISBN 9780199630530

内容説明

The recent explosion in the field of flow cytometry and the rapid increase in the number of machines being installed has resulted in many workers trying to cope with this new technology. This book offers practical guidance in preparing and analysing samples. It will also act as a first point of reference for established workers who wish to add to their repertoire of applications.

目次

  • N. Carter & E.W. Meyer Introduction to the principles of flow cytometry
  • M.G. Ormerod: An introduction to fluorescience technology
  • N.P. Carter: Measurement of cellular subsets using antibodies
  • M.G. Ormerod, G. Wilson, N. McNally, & M. Poot: Analysis of DNA
  • L.K. Borysiewicz, T.G Hoy, P.R. Imrie: Cell sorting
  • B.D. Young: Chromosome analysis and sorting
  • P.S. Rabinovitch, & C.H. June: Intracellular ionized calcium, membrane potential, and pH
  • H.J. Tenke: Flow cytometry of RNA content
  • J.V. Watson: Nuclear associated antigens
  • A.T.M. Vaughan, D.J. Gordon, D.J. Grdina, & A.E. Milner: Analysis of radiation and chemical damage in mammalian cells
  • J.V. Watson, C. Dive, & P. Workman: Measurement of dynamic cellular events
  • M.G. Ormerod: Further applications to cell biology

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