Gel electrophoresis of proteins : a practical approach
著者
書誌事項
Gel electrophoresis of proteins : a practical approach
（The practical approach series）
IRL Press at Oxford University Press, c1990
2nd ed
- : pbk
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注記
Includes bibliographical references and index
内容説明・目次
- 巻冊次
-
ISBN 9780199630745
内容説明
Here is a newly updated and expanded edition of a useful and popular guide. In addition to providing new information on topics covered in the first edition, this volume covers a number of major techniques which must be mastered by molecular biologists and biochemists. Every aspect of polyacrylamide gel electrophoresis is described, while valuable appendix materials bring the reader useful listings of suppliers' addresses, detection methods, and more.
目次
- One dimensional polyacrylamide gel electrophoresis, B.D.Hames
- Isoelectric focusing, P.G.Righetti, E.Gianazza, and C.Gelfi
- two-dimensional gel electrophoresis, D.Rickwood, J.A.A.Chambers and S.Spragg
- Immunoelectrophoresis, T.C.B g-Hansen
- Peptide mapping, A.Andrews. Appendices.
- 巻冊次
-
: pbk ISBN 9780199630752
内容説明
Since the first edition of this book, the electrophoretic analysis of proteins in polyacrylamide gels has continued to grow in importance as an essential research technique in the life sciences. While some techniques have changed only marginally, many new procedures and applications have arisen in the intervening years. This second edition seeks to reflect these changes. It also features a new design which provides enhanced readability. One-dimensional polyacrylamide gel electrophoresis is currently the most widely used form of the technique in all areas of the life sciences and so a greatly extended first chapter is devoted to this topic. This chapter also covers many of the recently developed methods for analysing gels, especially the different staining and blotting protocols. The subsequent chapters describe in great practical detail the other major gel electrophoretic techniques that are now in common use, including isoelectric focusing, with both conventional and immobilised pH gradients, two-dimensional gel electrophoresis, peptide mapping, and immunoelectrophoresis. This book is intended for researchers and postgraduates in molecular biology and biochemistry.
目次
- One dimensional polyacrylamide gel electrophoresis, B.D.Hames
- Isoelectric focusing, P.G.Righetti, E.Gianazza, and C.Gelfi
- two-dimensional gel electrophoresis, D.Rickwood, J.A.A.Chambers and S.Spragg
- Immunoelectrophoresis, T.C.B g-Hansen
- Peptide mapping, A.Andrews. Appendices.
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