Chromosome banding

著者

書誌事項

Chromosome banding

A.T. Sumner

Unwin Hyman, 1990

大学図書館所蔵 件 / 全21件

この図書・雑誌をさがす

注記

Includes bibliographical references (p. [349]-424) and index

内容説明・目次

内容説明

In the past 20 years since chromosome banding techniques came into general use, they have become essential tools in all fields of research concerned with chromosomes. Just as important, however, are the insights that banding has provided the organization of chromosomes. "Chromosome Banding" is a comprehensive work providing a source of methods and understanding of the mechanisms and biological significance of banding. After an historical introduction and a general account of chromosome organization, each class of banding technique is considered in turn, with a description of the methods, the mechanisms behind the methods, their biologial significance and their applications. Separate chapters cover the subjects of banding heteromorphisms and evolutionary studies, and the book concludes with a consideration of the implications of banding for chromosome organization.

目次

  • Part 1 The origins and evolution of banding methods: definition of banding
  • the origins and evolution of chromosome banding methods
  • the modern era of banding
  • the current state of chromosome banding. Part 2 Classification and nomenclature of chromosome bands: the classification of types of chromosome bands
  • classification of banding methods
  • chromosome band nomenclature. Part 3 The structure and composition of chromosome: introduction
  • the constituents of chromosomes
  • the assembly of chromosomal constituents into chromosomes
  • changes during chromosome condensation
  • effects of fixation on chromosomes. Part 4 C-banding and related methods: C-banding
  • G-11 banding
  • N-banding
  • methods for demonstrating heterochromatic bands using hydrochloric acid
  • silver staining of heterochromatin. Part 5 G-banding
  • definition of G-banding
  • G-banding methods
  • characteristics of G-bands
  • the mechanisms of G-banding
  • applications of G-banding. Part 6 R- and T-banding: introduction and definitions
  • methods for R- and T-banding
  • the nature of R- and T-bands
  • the mechanisms of R- and T-banding
  • applications of R- and T-banding. Part 7 Q-banding: Q-banding methods
  • the nature of Q-bands
  • mechanisms of Q-banding
  • applications of Q-banding. Part 8 Banding with fluorochromes other than quinacrine: a fluorochrome that shows no base specificity - ethidium
  • banding with fluorochromes showing specificity for A+T-rich DNA
  • banding with fluorochromes shwoing specificity for G+C-rich DNA
  • light-induced banding
  • concluding remarks. Part 9 Nucleolar organizers (NORs): the nature of organization of NORs and nucleoli
  • methods for selective staining of NORs
  • N-banding (Giemsa staining of NORs)
  • silver (Ag-NOR staining). Part 10 The kinetochores: staining methods for kinetochores. Part 11 Banding produced by treatment of living cells - condensation and replication banding: inhibition of condensation
  • replication banding - BrdU substitution
  • unclassified effects of agents which induce banding in living cells
  • general discussion. Part 12 Banding with nucleases: digestion of chromosomes with non-specific nucleases
  • digestion of chromosomes with restriction endonucleases
  • general remarks on banding with nucleases
  • a note on nomenclature. (Part Contents)

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