Flow cytometry and sorting

著者

書誌事項

Flow cytometry and sorting

editors, Myron R. Melamed, Tore Lindmo, Mortimer L. Mendelsohn

Wiley-Liss, c1990

2nd ed

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注記

Includes bibliographical references

内容説明・目次

内容説明

Revised and updated, the second edition of this text describes and evaluates - in greater detail than the first edition - the most recent practical applications of flow cytometry technique to basic cellular biological investigations and clinical research on human neoplasms. Ideal for the experienced researcher as well as the novice, this informative book offers state-of-the-art reviews of all aspects of flow cytometry including instrumentation, cell preparation and staining, applications in cell biology, and more. New articles highlight investigations of higher plants, the flow cytometry of microorganisms, and measurements of intracellular ionized calcium and membrane potential, illustrating the techniques of specimen preparation, measurement and analysis for each. New chapters examine applications of flow cytometry to medical genetics, genetic toxicology and ultrasensitive analysis of molecules in solution. The second edition goes beyond the traditional analysis of DNA histograms with BrdU incorporation and DNA denaturability to identify and analyze the cell cycle more precisely. New or rewritten chapters discuss the importance of cytometry for measurements of nucleic acids, chromatin and DNA, and cover the cytometry of sperm and the cytopathic effects of viruses.

目次

  • Cytophysical Methods
  • Cell Preparation
  • Cytochemical Methods
  • Analysis of Measurements
  • Cell Biology
  • Hematology and Immunology
  • Applications in Oncology
  • Index.

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