Peptide hormone secretion : a practical approach
著者
書誌事項
Peptide hormone secretion : a practical approach
（The practical approach series）
IRL Press at Oxford University Press, c1990
- : pbk
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注記
Includes bibliographical references and index
内容説明・目次
- 巻冊次
-
ISBN 9780199630684
内容説明
In this the first of two volumes, attention is focused on procedures used in the isolation of polypeptide hormones and their indentification and structural analysis; the development of sensitive bioassay and immunoassay techniques; methods in current use for tissue localization of peptide hormones and their encoding MRNAs; and techniques for characterization of peptide hormone receptors. Wherever possible, the general principles and strategies on which the methods are based are given so that the techniques will have a wide application to biologically active peptides involved in neurotransmission, growth regulations, differentiation, and vasoregulation. This and the second volume "Peptide Hormone Action" are also published together as a two-volume set.
目次
- Peptide hormone purification, C.McLean
- electrophoretic and immunoblotting methods, P.Jackson
- radioimmunoassay, R.Edwards
- immunometric assays, K.Siddle
- the "In Vitro" bioassay of peptide hormones, W.R.Robertson and S.P.Bidey
- "In Situ" hybridization for the localization and quantification of peptide mRNAs, P.C.Emson et al
- localization of peptide hormones by light and electron microscopy, A.Clark and D.Pow
- methods for the measurement of insulin secretion, W.J.Malaisse et al
- cell biology of secretion, K.W.Brocklehurst and H.Pollard
- cell permeabilization, P.E.R.Tatham and B.Gomperts
- the molecular biology of polypeptide hormone expression and biosynthesis, D.S.W.Boam et al
- regulation of the biosynthesis and processing of polypeptide hormones J.C.Hutton et al.
- 巻冊次
-
: pbk ISBN 9780199630691
内容説明
In this the first of two volumes, attention is focused on procedures used in the isolation of polypeptide hormones and their indentification and structural analysis; the development of sensitive bioassay and immunoassay techniques; methods in current use for tissue localization of peptide hormones and their encoding MRNAs; and techniques for characterization of peptide hormone receptors. Wherever possible, the general principles and strategies on which the methods are based are given so that the techniques will have a wide application to biologically active peptides involved in neurotransmission, growth regulations, differentiation, and vasoregulation. This and the second volume "Peptide Hormone Action" are also published together as a two-volume set.
目次
- C. McLean: Peptide hormone purification
- P. Jackson: Electrophoretic and immunoblotting methods
- R. Edwards: Radioimmunoassay
- K. Siddle: Immunometric assays
- W.R. Robertson: The In vitro bioassay of peptide hormones
- P. C. Emson, D.J. Nunez, & A.P. Davenport: In situ hybridization for the localization and quantification of peptide mRNAs
- A. Clark & D. Pow: Localization of peptide hormones by light and electron microscopy
- W.J. Malaisse, V. LeClerq-Meyer, & F. Malaisse-Lagae: Methods for the measurement of insulin secretion
- K.W. Brocklehurst & H. Pollard: Cell biology of secretion
- P.E.R. Tatham & B. Gomperts: Cell permeabilization
- D.S.W. Boam, K. Docherty, K.I.J. Shennan, & A. Clark: The molecular biology of polypeptide hormone expression and biosynthesis
- J.C. Hutton, E.M. Bailyes, C.J. Rhodes, & P.C. Guest: Regulation of the biosynthesis and processing of polypeptide hormones
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