Techniques in HIV research

著者

書誌事項

Techniques in HIV research

edited by Anna Aldovini and Bruce D. Walker

Stockton Press, c1990

  • : U.S
  • : U.K

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内容説明・目次

内容説明

A comprehensive compilation of research techniques necessary for investigating the virology, immunology and molecular biology of HIV-1. Protocols are also provided which represent state of the art approaches to a wide spectrum of HIV related issues.

目次

  • Part 1 Antibody detection in patient's serum: ELISA assay
  • western blot analysis. Part 2 Virus detection in pathological specimen: p24 assay
  • polymerase chain reaction (PCR)
  • in situ hybridization
  • southern blot analysis. Part 3 Virus isolation and production: isolation from periperal blood
  • isolation from tissues
  • virus production in stable cell lines. Part 4 Virus titration: on MT4 cells
  • on HeLa cells
  • on C81-66
  • on SUP T1. Part 5 Virus detection in cell lines: reverse transcriptase assay
  • p24 assay
  • immunofluorescence (IF)
  • cythopathic effect (CPE)
  • radioimmunoprecipitation (RIP). Part 6 Virus purification and storage. Part 7 Proviral DNA cloning: recombinant DNA libraries
  • polymerase chain reaction (PCR). Part 8 Tranfection of biologically active molecular clones: DEAE dextran
  • electroporation
  • CaPO4 transfection
  • lipofection
  • selection of stably transfected cell lines. Part 9 Host immune response: neutralizing antibodies
  • ADCC/CMC
  • cytotoxity - CTL
  • proliferative response. Part 10 Cytokine assays. Part 11 Antiviral drugs assays. Appendices: source of reagents
  • biosafey issues.

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