An introduction to radioimmunoassay and related techniques
Author(s)
Bibliographic Information
An introduction to radioimmunoassay and related techniques
(Laboratory techniques in biochemistry and molecular biology, v. 6,
Elsevier , Sole distributors for the U.S.A. and Canada, Elsevier Science Pub. Co., 1990
4th rev. ed
- : library ed
- : pocket ed
Available at 26 libraries
  Aomori
  Iwate
  Miyagi
  Akita
  Yamagata
  Fukushima
  Ibaraki
  Tochigi
  Gunma
  Saitama
  Chiba
  Tokyo
  Kanagawa
  Niigata
  Toyama
  Ishikawa
  Fukui
  Yamanashi
  Nagano
  Gifu
  Shizuoka
  Aichi
  Mie
  Shiga
  Kyoto
  Osaka
  Hyogo
  Nara
  Wakayama
  Tottori
  Shimane
  Okayama
  Hiroshima
  Yamaguchi
  Tokushima
  Kagawa
  Ehime
  Kochi
  Fukuoka
  Saga
  Nagasaki
  Kumamoto
  Oita
  Miyazaki
  Kagoshima
  Okinawa
  Korea
  China
  Thailand
  United Kingdom
  Germany
  Switzerland
  France
  Belgium
  Netherlands
  Sweden
  Norway
  United States of America
-
Obihiro University of Agriculture and Veterinary Medicine Library図
: library ed464/C34020366019
-
National Institutes of Natural Sciences Okazaki Library and Information Center図
: library ed464.07/L11/6-29215990210
Note
Includes bibliographical references and index
Description and Table of Contents
Description
Immunoassay procedures have become one of the most important techniques in present-day diagnostic medicine. The move to non-radioactive tracers, the switch from polyclonal to monoclonal antibodies, and from labelled antigens to labelled antibodies are fully acknowledged and reflected in this volume. The book describes the general principles of immunoassay and the technical requirements and characteristics of such assays. Whenever appropriate, specific practical examples are given which describe in detail the preparation of materials and the conduct of an assay. Both principles and practice are illustrated by a wealth of diagrams and illustrations. An Introduction to Radioimmunoassay and Related Techniques is designed as an introductory text for the staff of clinical or research laboratories who conduct or intend to conduct such techniques, and will also be of great value to the clinicians who make use of such services. The emphasis is on general principles with practical illustrations rather than a detailed and encyclopaedic review of the literature.
Table of Contents
(Subsections not included) Chapter 1: 1.1 Introduction. 1.2 Terminology. 1.3 Early development of radioimmunoassay. 1.4 Basic principles of binding assays. 1.5 Binder dilution curves and standard curves. 1.6 Methods for plotting the standard curve. 1.7 The measurement of K value. Chapter 2: 2.1 The need for purified ligand. 2.2 Availability of pure ligand. 2.3 Dissimilarity between purified ligand and endogenous ligand. 2.4 Standards. 2.5 Storage of materials used in binding assays. Chapter 3: 3.1 Radioactive isotopes. 3.2 Counting of radioactive isotopes. 3.3 Choice of counter. 3.4 Some practical aspects of isotope counting. 3.5 Essential characteristics of a tracer. 3.6 Preparation of tracers. 3.7 Iodinated tracers. 3.8 Variations on the use of radiolabelled tracers. Chapter 4: 4.1 Particle labels. 4.2 Enzyme labels (enzymoimmunoassay, EIA). 4.3 Fluorescent labels (fluoroimmunoassay, FIA). 4.4 Luminescent labels. 4.5 Advantages and disadvantages of non-isotopic labels in immunoassays. 4.6 Conclusions: the place of non-isotopic binding assays. Chapter 5: 5.1 Antibodies and the immune response. 5.2 Preparation of Antisera for use in RIA. 5.3 'Monoclonal' antibodies. 5.4 Cell receptors. 5.5 Circulating binding proteins. 5.6 Assays for the detection of endogenous antibodies, circulating binding proteins and receptors. Chapter 6: 6.1 Efficiency of separation procedures. 6.2 Practicality of separation procedures. 6.3 Methods of separation of bound and free ligand. 6.4 Immunometric techniques. Chapter 7: 7.1 General aspects of extraction procedures. 7.2 Extraction using particulate adsorbents. 7.3 Extraction with immunoadsorbents. 7.4 Extraction with organic solvents. 7.5 Dissociation procedures. 7.6 Measurement of 'free' hormone or drug. 7.7 Conclusions - the elimination of extraction procedures. 7.8 Sample collection and transport for immunoassay. Chapter 8: 8.1 Calculation of results by manual extrapolation. 8.2 Data transformation of the standard curve. 8.3 The logit transformation. 8.4 Identification of outliers. 8.5 Estimation of confidence limits to the result of an unknown. 8.6 Computer calculation of results. 8.7 Calculation of results of labelled antibody assays. 8.8 Presentation of results. Chapter 9: 9.1 Definition of sensitivity. 9.2 Methods of increasing the sensitivity of a labelled antigen immunoassay. 9.3 Methods of increasing the sensitivity of a labelled antibody assay (immunometric assays). 9.4 Methods of decreasing the sensitivity of an assay. 9.5 The low-dose hook effect. 9.6 Targeting of binding assays - the importance of ranges. 9.7 Optimisation of an assay by theoretical analysis. 9.8 Conclusions. Chapter 10: 10.1 Definition of specificity. 10.2 Specific non-specificity. 10.3 Non-specific non-specificity. Chapter 11: 11.1 Definitions. 11.2 Factors affecting precision. 11.3 Quality control to monitor the precision of a binding assay. 11.4 Practical use of a quality-control scheme. 11.5 External quality control schemes.
by "Nielsen BookData"