Cryopreparation of thin biological specimens for electron microscopy : methods and applications
著者
書誌事項
Cryopreparation of thin biological specimens for electron microscopy : methods and applications
（Microscopy handbooks, 21）
Oxford University Press, c1990
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注記
Bibliography: p. 93-104
Includes index
内容説明・目次
内容説明
This practical handbook describes the basic principles of electron microscopical cryotechniques. Prior knowledge of electron microscopy is assumed, but not of cryotechniques. The basic principles of electron microscopical cryotechniques are clearly explained in this useful guide. The handbook is written primarily for researchers, postgraduate students and technicians who are familiar with the basics of biological electron microscopy, but have little experience of cryotechniques. It provides firm practical gujidelines for those techniques where the cryofixed specimen is sectioned or otherwise presented as a thin film for examination in the TEM, devoting particular attention to methods of contemporary interest.
目次
- Water, cells, and electron microscopy
- freezing methods
- cryo-ultramicrotomy
- the transfer of cryosections to the TEM for morphological examination and X-ray microanalysis
- imaging and analysis of frozen-hydrated specimens in the TEM - vitrified thin films and cryosections
- the use of ultrathin cryosections in immunocytochemistry
- other low-temperature methods
- safety considerations and concluding remarks.
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