Molecular motors and the cytoskeleton
著者
書誌事項
Molecular motors and the cytoskeleton
（Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan, v. 196,
Academic Press, c1991-c1998
- [Part A]
- Part B
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注記
Includes bibliographical footnotes and indexes
内容説明・目次
- 巻冊次
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[Part A] ISBN 9780121820978
内容説明
This volume attempts to describe the purification and assay of the diverse group of proteins involved in otoplasmic organization. Some of the more promising attempts to apply techniques of molecular biology, genetics, and molecular cytochemistry to these proteins are discussed.
目次
Preparation of Myosin- and Actomyosin-Related Proteins:H. Swanljung-Collins and J.H. Collins, Rapid, High-Yield Purification of Intestinal Brush Border Myosin I.T.J. Lynch, H. Brzeska, I.C. Baines, and E.D. Korn, Purification of Myosin I and Myosin I Heavy Chain Kinase from Acanthamoeba castellanii.Q.G. Medley, S.-F. Lee, and G.P. C~acot~aae, Purification and Characterization of Myosin II Heavy Chain Kinase A from Dictyostelium.M.A. Conti and R.S. Adelstein, Purification and Properties of Myosin Light Chain Kinases.L.J. Wuestehube, D.W. Speicher, A. Shariff, and E.J. Luna, F-Actin Affinity Chromatography of Detergent-Solubilized Plasma Membranes: Purification and Initial Characterization of Ponticulin from ~Dictyostelium discoideum.J.R. Glenney, Jr. , Purification of Calpactins I and II and Isolation of N-Terminal Tail of Calpactin I.A.R. Bresnick and J. Condeelis, Isolation of Actin-Binding Proteins from Dictyostelium discoideum.M. Fechheimer and R. Furukawa, Preparation of 30,000-Da Actin Cross-Linking Protein from Dictyostelium discoideum.P.A. Janmey, Polyproline Affinity Method for Purification of Platelet Profilin and Modification with Pyrene-Maleimide.M. Rozycki, C.E. Schutt, and U. Lindberg, Affinity Chromatography-Based Purification of Profilin: Actin.M.R. Bubb and E.D. Korn, Purification of Actobindin from Acanthamoeba castellanii.J.R. Bamburg, L.S. Minamide, T.E. Morgan, S.M. Hayden, K.A. Giuliano, and A. Koffer, Purification and Characterization of Low-Molecular-Weight Actin-Depolymerizing Proteins from Brain and Cultured Cells.J.F. Casella and J.A. Cooper, Purification of Cap Z from Chicken Skeletal Muscle.Preparation of Microtubules, Microtubule-Associated Proteins, and Microtubule Motors:M.C. Wagner, K.K. Pfister, S.T. Brady, and G.S. Bloom, Purification of Kinesin from Bovine Brain and Assay of Microtubule-Stimulated ATPase Activity.D.D. Hackney, Isolation of Kinesin Using Initial Batch Ion Exchange.B.M. Paschal, H.S. Shpetner, and R.B. Vallee, Purification of Brain Cytoplasmic Dynein and Characterization of Its in Vitro Properties.H.S. Shpetner and R.B. Vallee, Purification and Characterization of Dynamin.A.G. Moss, J.-L. Gatti, S.M. King, and G.B. ~Witman, Purification and Characterization of Salmo gairdneri Outer Arm Dynein.A.T. Hastie, Purification and Characterization of Dynein from Pig Tracheal Cilia.D.B. Murphy, Purification of Tubulin and Tau from Chicken Erythrocytes: Tubulin Isotypes and Mechanisms of Microtubule Assembly.C.A. Collins, Reversible Assembly Purification of Taxol-Treated Microtubules.S.J. Chapin and J.C. Bulinski, Preparation and Functional Assay of Pure Populations of Tyrosinated and Detyrosinated Tubulin.M. LeDizet and G. Piperno, Detection of Acetylated ~ga-Tubulin by Specific Antibodies.E. Aamodt, Isolation of Microtubules, Adligin, and Other Microtubule-Associated Proteins from Caenorhabditis elegans.D. Robinson, P. Beattie, T. Sherwin, and K. Gull, ~Microtubules, Tubulin, and Microtubule-Associated Proteins of Trypanosomes.Molecular and Genetic Approaches:K.G. Miller, C.M. Field, B.M. Alberts, and D.R. Kellogg, Use of Actin Filament and Microtubule Affinity Chromatography to Identify Proteins That Bind to the Cytoskeleton:T.T. Egelhoff, M.A. Titus, D.J. Manstein, K.M. Ruppel, and J.A. Spudich, Molecular Genetic Tools for Study of the Cytoskeleton in Dictyostelium.E. Wallraff and G. Gerisch, Screening for Dictyostelium Mutants Defective in Cytoskeletal Proteins by Colony Immunoblotting.R. Kamiya, Selection of Chlamydomonas Dynein Mutants.T.C. Huffaker and A.P. Bretscher, Cloning and Analyzing Genes Encoding Cytoskeletal Proteins in Yeast Saccharomyces cerevisiae.E. McNally, R. Sohn, S. Frankel, and L. Leinwand, Expression of Myosin and Actin in Escherichia coli.G.-Q. Xu and S.E. Hitchcock-DeGregori, Synthesis of cDNAs from Synthetic Oligonucleotides Using Troponin C as an Example.Mechanochemical Properties of Motor Proteins:S.J. Kron, Y.Y. Toyoshima, T.Q.P. Uyeda, and J.A. Spudich, Assays for Actin Sliding Movement over Myosin-Coated Surfaces.S. Ishijima and G.B. Witman, Demembranation and Reactivation of Mammalian Spermatozoa from Golden Hamster and Ram.I.R. Gibbons and G. Mocz, Photocatalytic Cleavage of Proteins with Vanadate and Other Transition Metal Complexes.C.R. Cremo, J.C. Grammer, and R.G. Yount, Vanadate-Mediated Photocleavage of Myosin.S.M. King, H. Kim, and B.E. Haley, Strategies and Reagents for Photoaffinity Labeling of ~Mechanochemical Proteins.Chemical Modifications and Assembly Assays:S.R. Heidemann, Microtubule Polarity Determination Based on Formation of Protofilament Hooks.A. Hyman, D. Drechsel, D. Kellogg, S. Salser, K. Sawin, P. Steffen, L. Wordeman, and T. Mitchison, Preparation of Modified Tubulins.J. Condeelis and A.L. Hall, Measurement of Actin Polymerization and Cross-Linking in Agonist-Stimulated Cells.Y.-L. Wang, Preparation and Characterization of Tetramethylrhodamine-Labeled Myosin.K.L. Vikstrom, R.K. Miller, and R.D. Goldman, Analyzing Dynamic Properties of Intermediate Filaments.Each chapter includes references.Author Index.Subject Index.
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Part B ISBN 9780121821999
内容説明
This Methods in Enzymology volume continues the description (initiated in Volume 85) of the purification and assay of this diverse group of proteins involved in cytoplasmic organization. It discusses some of the more promising attempts to apply techniques of molecular biology genetics and molecular cytochemistry to these proteins.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
目次
Analysis of Actomyosin-Related Systems:
R.E. Cheney, Purification and Assay of Myosin V.
K. Ayscough, Use of Latrunculin-A, An Actin Monomer Binding Drug.
M.R. Bubb and I. Spector, Use of the F-Actin Binding Drugs, Misakinolide A and Swinholide A.
E.J. Luna, F-Actin Blot Overlays.
J.F. Kelleker, R.D. Mullins, and T.D. Pollard, Purification and Assay of the Arp2/3 Complex from Acanthamoeba castellani.
M.D. Welch and T.J. Mitchison, Purification and Assay of the Platelet Arp2/3 Complex.
K.L. Schmeichel, B.E. Stronach, and M.C. Beckerle, Purification and Assay of Zyxin.
C.E. Turner and M.C. Brown, Purification and Assays for Paxillin.
J. Lacoste, A. Ma, and J.T. Parsons, Assay and Purification of Focal Adhesion Kinase.
T. Jarchau, T. Mund, M. Reinhard, and U. Walter, Purification and Assays of VASP.
J.A. Theriot and D.C. Fung, Listeria monocytogenes-based Assays for Actin Assembly Factors.
Analysis of Microtubule-Related Systems:
A. Desai and T.J. Mitchison, Preparation and Characterization of Caged Fluorescein Tubulin.
D. Meyer, D.R. Rines, A. Kashina, and J.M. Scholey, Purification of Novel Kinesins from Embryonic Systems.
D.W. Pierce and R.D. Vale, Assaying Processive Movement of Kinesin by Fluorescence Microscopy.
J.B. Bingham, S.J. King, and T.A. Schroer, Purification of Dynactin and Dynein from Brain Tissue.
M.P. Sheetz and J. Kumar, Isolation and Characterization of Kinectin.
G.S. Diamantopoulos, T.E. Kreis, and J.E. Rickard, Purification and Assay of CLIP-170.
F. McNally, Purification and Assay of the Microtubule-Severing Protein, Katanin.
Y. Zheng, M.L. Wong, B. Alberts, and T. Mitchison, Purification and Assay of gTubulin Ring Complex.
M. Blomberg-Wirschell and S.J. Doxsey, Rapid Isolation of Centrosomes.
G.A. Orr, S. Rao, C.S. Swindell, D.G.I. Kingston, and S.B. Horwitz, Photoaffinity Labeling Approach to Map Taxol-Binding Site on the Microtubule.
M.A. Jordan and L.Wilson, Use of Drugs to Study Role of Microtubule Assembly Dynamics in Living Cells.
Other Cytoskeletal Systems:
K. Oegema, A. Desai, M.L. Wong, T.J. Mitchison, and C.M. Field, Purification and Assay of A Septin Complex from Drosophila Embryos.
A. Mukherjee and J. Lutkenhaus, Purification, Assembly and Localization of FtsZ.
C. Lu and H.P. Erickson, Purification and Assembly of FtsZ. Cell-Free and Genetic Systems:
E.D. Salmon, E. Yeh, S. Shaw, B. Skibbens, and K. Bloom, High Resolution Video and Digital Enhanced Differential Interference Contrast Light Microscopy of Cell Division in Budding Yeast.
D.A. Compton, Production of M-phase and I-phase Extracts from Mammalian Cells.
V.J. Allan, Organelle Motility and Membrane Network Formation in Metaphase and Interphase Cell-Free Extracts.
C. McGoldrick and M. Sheetz, Organelle Motility and Membrane Network Formation from Cultured Mammalian Cells.
S.L. Rogers, I.S. Tint, and V.I. Gelfand, An in Vitro Motility Assay for Melanophore Pigment Organelles.
J.K. Burkhardt, Purification of Phagosomes and Assays for Microtubule Binding.
J. Scheel and T.E. Kreis, Magnetic Bead Assay for Characterization of Microtubule-Membrane Interactions.
L. Haimo, Reactivation of Vesicle Transport in Lysed Teleost Melanophores.
M.P. Koonce, U. Euteneuer, and M. Schliwa, Reactivatable Cell Models of Giant Ameba, Reticulomyxa.
Force Production Assays:
R.J. Baskin, Design and Use of the Centrifuge Microscope to Assay Force Production.
Y.J. Han and J.R. Sellers, Motility Assays on Molluscan Native Thick Filaments.
A.D. Mehta, J.T. Finer, and J.A. Spudich, Use of Optical Traps in Single Molecule Study on Nonprocessive Biological Motors.
K. Visscher and S.M. Block, Versatile Optical Traps with Feedback Control.
Y. Wang and R.J. Pelham, Jr., Preparation of a Flexible, Porous Polyacrylamide Substrate for Mechanical Studies of Cultured Cells.
T. Oliver, K. Jacobson, and M. Dembo, Design and Use of Substrata to Measure Traction Forces Exerted by Cultured Cells. General Methods:
C.M. Field, K. Oegema, Y. Zheng, T.J. Mitchison, and C.E. Walczak, Purification of Cytoskeletal Proteins Using Peptide Antibodies.
J.E. Eriksson, D.M. Toivola, C. Sahlgren, A. Mikhailov, and A.S. Harmala-Brasken, Strategies to Assess Phosphoprotein Phosphatase and Protein Kinase-Mediated Regulation of the Cytoskeleton.
T.M. Svitkina and G.G. Borisy, Correlative Light and Electron Microscopy of the Cytoskeleton of Cultured Cells.
Subject Index.
Author Index.
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