Immunoenzymatic techniques
著者
書誌事項
Immunoenzymatic techniques
（Techniques in immunology, v. 1）
Elsevier , Sole distributors for the U.S.A. and Canada, Elsevier Science Pub. Co., 1990
- タイトル別名
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Techniques immunoenzymatiques
- 統一タイトル
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Techniques immunoenzymatiques
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注記
Outcome of a technological workshop organized by the French Society of Immunology
Translation of: Techniques immunoenzymatiques
Includes bibliographical references
Includes index
内容説明・目次
内容説明
This practical manual is translated from the highly successful French publication `Techniques Immunoenzymatiques', published by INSERM for the Societe d'Immunologie. A useful bench-tool describing a series of experimental procedures for the application of immunoenzymatic methods and the preparation of reagents employed. Attractive features include: - clear, concise descriptions of underlying principles - extensive use of technical notes to facilitate application
目次
Content headings: List of authors and contributors. Preface. Acknowledgements. Introduction. Chapter 1. Purification of antibodies. Chemical methods. Immunochemical methods. Use of protein A. Preparation of the F(ab') 2 and Fab fragments of antibodies. Chapter 2. Coupling of antibodies to enzymes. Principle. Coupling of enzymes to antibodies by means of glutaraldehyde. Coupling of peroxidase by means of sodium periodate. Coupling of enzyme to antibodies by means of glutaraldehyde. Coupling of peroxidase by means of sodium periodate. Coupling of enzyme to antibodies by means of p -benzoquinone. Purification of conjugates. Chapter 3. Quantitative enzyme immunoassays. Principle. Specification of materials. Methodology. Examples. Expression of the results. Chapter 4. Enzyme-immunocytochemical methods. Principle. Specification of materials. Methodology. Examples. General comments. Chapter 5. Detection of proteins after transfer to nitrocellulose. Principle. Methodology. Chapter 6. Use of monoclonal antibodies. Preparation. Coupling of antibodies to labels. ELISA. Enzyme-immunocytochemical methods. Chapter 7. Amplification systems for immunoenzymatic methods. Use of a fluorogenic substrate for beta-galactosidase. Use of the avidin-biotin system. Use of the peroxidase-anti-peroxidase system. Appendix. List of suppliers. Subject index.
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