書誌事項

Cell separation science and technology

Dhinakar S. Kompala, editor, Paul Todd, editor

(ACS symposium series, 464)

American Chemical Society, 1991

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注記

"Developed from a symposium sponsored by the Divisions of Industrial and Engineering Chemistry, Inc., and Biochemical Technology at the 199th National Meeting of the American Chemical Society, Boston, Massachusetts, April 22-27, 1990"

Includes bibliographical references and indexes

内容説明・目次

内容説明

This new volume provides theoretical and practical information on the state of the science of cell separation, including applications to biotechnology. Presentations from the world's leading authorities cover the four principal modern methods: flow sorting and optical methods; sedimentation and flow fractionation; affinity adsorption and extraction methods; and electrophoresis and magnetic methods. An introductory chapter describes the rationale for separating living cells, the parameters within which separation methods are evaluated, and some physical characteristics of each of the methods reported in the volume. Readers will be able to choose, evaluate, and in some cases, build cell separation procedures and equipment for their own purposes based on the contents of the book, which also covers many applications of each method.

目次

  • Separation of Living Cells
  • High-Resolution Separation of Rare Cell Types
  • Rare-Earth Chelates as Fluorescent Markers in Cell Separation and Analysis
  • Automated Cell Separation Techniques Based on Optical Trapping
  • Separation Techniques Used To Prepare Highly Purified Chromosome Populations: Sedimentation, Centrifugation, and Flow Sorting
  • Separation of Cells by Sedimentation
  • High-Capacity Separation of Homogeneous Cell Subpopulations by Centrifugal Elutriation
  • Cell Separations Using Differential Sedimentation in Inclined Settlers
  • Separation of Cells by Field-Flow Fractionation
  • Separation of Cells and Measurement of Surface Adhesions Forces Using a Hybrid of Field-Flow Fractionation and Adhesion Chromatography
  • High-Capacity Cell Separation by Affinity Selection on Synthetic Solid-Phase Matrices
  • Factors in Cell Separation by Partitioning in Two-Polymer Aqueous-Phase Systems
  • Population Heterogeneity in Blood Neutrophils Fractionated by Continuous Flow Electrophoresis (CFE) and by Partitioning in Aqueous Polymer Two-Phase Systems (PAPS)
  • Separation of Lymphoid Cells Using Combined Countercurrent Elutriation and Continuous Flow Electrophoreses
  • Comparison of Methods of Preparative Cell Electrophoresis
  • Separation of Small-Cell Lung Cancer Cells from Bone Marrow Using Immunomagnetic Beads
  • Analytical- and Process-Scale Cell Separation with Bioreceptor Ferrofluids and High-Gradient Magnetic Separation

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