Mammalian cell biotechnology : a practical approach
Author(s)
Bibliographic Information
Mammalian cell biotechnology : a practical approach
（The practical approach series）
IRL Press, c1991
- : pbk
Available at / 16 libraries
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University Library for Agricultural and Life Sciences, The University of Tokyo図
613.6:B965019165835
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Note
Includes bibliographical references
Description and Table of Contents
- Volume
-
ISBN 9780199632077
Description
The development of mammalian cell biotechnology has led to an extensive range of commercially-valuable prophylactic, diagnostic and therapeutic compounds which can now be produced routinely by cell culture. This book details the principles and practical techniques upon which this technological development is based. The ten chapters are designed to cover the basic principles and techniques which can be applied to maximize the productivity of cell cultures. Media optimization and the use of serum-substitutes or growth factors are explained. Methods of genetic manipulation of cells are explored as well as the techniques for maximizing specific protein expression. Chapters are included to explain the design and control of animal cell fermentation processes and the means of extraction of desired products by downstream processing. Finally, a chapter is included to explain the strategies for the application of these techniques to the production of specific compounds which have commercial value. This book should be of value to both the research student of biotechnology in academic institutions and to the industrialist who needs to maximize productivity from mammalian cell processes.
The detailed principles and techniques described in the book should advance the understanding and realization of high productivity from mammalian cell cultures.
Table of Contents
- The characteristics and growth of cultured cells, M.Butler
- serum and its fractionation, A.MacLeod
- growth factors, N.Jenkins
- genetic engineering of animal cells, C.MacDonald
- protein expression and processing, A.Dickson
- hybridomas - production and selection, C.Harbour and A.Fletcher
- bioreactor design, A.Handa-Corrigan
- monitoring and control of bioreactors, D.Kilburn
- downstream processing protein recovery, A.Lyddiatt
- products from animal cells, B.Griffiths.
- Volume
-
: pbk ISBN 9780199632091
Description
The development of mammalian cell biotechnology has led to an extensive range of commercially-valuable prophylactic, diagnostic and therapeutic compounds which can now be produced routinely by cell culture. This book details the principles and practical techniques upon which this technological development is based. The ten chapters are designed to cover the basic principles and techniques which can be applied to maximize the productivity of cell cultures. Media
optimisation and the use of serum-substitutes or growth factors are explained. Methods of genetic manipulation of cells are explored as well as the techniques for maximizing specific protein expression.
Chapters are included to explain the design and control of animal cell fermentation processes and the means of extraction of desired products by downstream processing. Finally, a chapter is included to explain the strategies for the application of these techniques to the production of specific compounds which have commercial value.
This book will be of value to both the research student of biotechnology in academic institutions and to the industrialist who needs to maximize productivity from mammalian cell processes. The detailed principles and techniques described in the book should advance the understanding and realization of high productivity from mammalian cell cultures.
Table of Contents
- M. Butler: The characteristics and growth of cultured cells
- A. MacLeod: Serum and its fractionation
- N. Jenkins: Growth factors
- C. MacDonald: Genetic engineering of animal cells
- A. Dickson: Protein expression and processing
- C. Harbour & A. Fletcher: Hybridomas: production and selection
- A. Handa-Corrigan: Bioreactor design
- D. Kilburn: Monitoring and control of bioreactors
- A. Lyddiatt: Downstream processing protein recovery
- B. Griffiths: Products from animal cells
by "Nielsen BookData"
