Essential molecular biology : a practical approach

著者

書誌事項

Essential molecular biology : a practical approach

edited by T.A. Brown

（The practical approach series）

IRL Press at Oxford University Press, c1991

  • v. 1
  • v. 2
  • : pbk. : v. 1
  • : pbk. : v. 2

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注記

Includes bibliographical references and index

内容説明・目次

巻冊次

v. 1 ISBN 9780199631100

内容説明

Like most scientific disciplines, the techniques for studying genes and DNA are complicated and difficult for the novice to understand. This book provides an introduction to practical molecular biology and is aimed specifically at students and other research workers who have no prior experience of the subject. In this part of the volume the fundamental techniques for handling and cloning DNA are described, with the emphasis on sound technical instruction, together with theoretical background and trouble-shooting guidance. The aim is to give the reader a sufficiently strong knowledge of these general techniques to allow the procedures to be used with confidence in specific research applications.

目次

  • Microbiological techniques for molecular biology, B.W.Bainbridge
  • purification of DNA, P.Towner
  • purification of RNA, M.Wilkinson
  • electrophoresis of nucleic acids, A.T.Andrews
  • recovery of DNA from electrophoresis gels, P.Towner
  • construction of recombinant DNA molecules, F.Cannon and R.Powell
  • generation and identification of recombinant clones, T.A.Brown
  • survey of cloning vectors of both plasmids and phages, P.H.Pouwels.
巻冊次

: pbk. : v. 1 ISBN 9780199631117

内容説明

The two volumes of Essential Molecular Biology: A Practical Approach provide clear theoretical and practical guidance to the fundamental techniques of recombinant DNA analysis. Although of particular value to research workers entering the field for the first time, the protocols described will also be of interest to experienced researchers. Volume I: concentrates on the preparation, purification and handling of nucleic acids, and includes chapters on microbiological procedures and the construction and use of recombinant DNA molecules. Each chapter is written in an accessible style by an acknowledged expert in that field with the emphasis on providing sound technical instruction, in combination with the necessary theoretical background and useful troubleshooting guidance. This book thus brings powerful techniques at the forefront of molecular biology research within reach of any researcher. Together, the two volumes provide a comprehensive molecular biology manual tailored specially for the novice.

目次

  • T.A. Brown: The essential techniques in molecular biology
  • B.W. Bainbridge: Microbiological techniques for molecular biology
  • P. Towner: Purification of DNA
  • M. Wilkinson: Purification of RNA
  • A.T. Andrews: Electrophoresis of nucleic acids
  • P. Towner: Recovery of DNA from electrophoresis gels
  • F. Gannon, & R. Powell: Construction of recombinant DNA molecules
  • T.A. Brown: Generation and identification of recombinant clones
  • P.H. Pouwels: Survey of cloning vectors of both plasmids and phages
巻冊次

v. 2 ISBN 9780199631124

内容説明

Like most scientific disciplines, the techniques for studying genes and DNA are complicated and difficult for the novice to understand. This book provides an introduction to practical molecular biology and is aimed specifically at students and other research workers who have no prior experience of the subject. In this part of the volume the more specialized procedures for gene identification and characterization are covered, with the emphasis on sound technical instruction, together with theoretical background and trouble-shooting guidance. The aim is to give the reader a sufficiently strong knowledge of these general techniques to allow the procedures to be used with confidence in specific research applications.

目次

  • Strategies for gene isolation and analysis, T.A.Brown
  • construction of genomic libraries in lambda and cosmid vectors, Anna-Maria Frischauf
  • the establishment of cDNA libraries in lambda gt10, U.Gubler and A.O.Chua
  • nucleic acid labelling and detection, C.R.Mundy et al
  • immobilization of nucleic acids and hybridization analysis, N.J.Dyson
  • DNA sequencing, C.J.Howe
  • DNA amplification by the polymerase chain reaction, T.D.Kocher and A.C.Wilson
  • DNA-protein interactions, J.H.Spencer
  • sequence analysis of RNA, H.Beier and H.J.Gross
  • methods for mapping transcribed DNA sequences, C.P.Smith.
巻冊次

: pbk. : v. 2 ISBN 9780199631131

内容説明

Like most scientific disciplines, the techniques for studying genes and DNA are complicated and difficult for the novice to understand. This book provides an introduction to practical molecular biology and is aimed specifically at students and other research workers who have no prior experience of the subject. In this part of the volume the more specialized procedures for gene identification and characterization are covered, with the emphasis on sound technical instruction, together with theoretical background and trouble-shooting guidance. The aim is to give the reader a sufficiently strong knowledge of these general techniques to allow the procedures to be used with confidence in specific research applications.

目次

  • Strategies for gene isolation and analysis, T.A.Brown
  • construction of genomic libraries in lambda and cosmid vectors, Anna-Maria Frischauf
  • the establishment of cDNA libraries in lambda gt10, U.Gubler and A.O.Chua
  • nucleic acid labelling and detection, C.R.Mundy et al
  • immobilization of nucleic acids and hybridization analysis, N.J.Dyson
  • DNA sequencing, C.J.Howe
  • DNA amplification by the polymerase chain reaction, T.D.Kocher and A.C.Wilson
  • DNA-protein interactions, J.H.Spencer
  • sequence analysis of RNA, H.Beier and H.J.Gross
  • methods for mapping transcribed DNA sequences, C.P.Smith.

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