Molecular biology and biotechnology
著者
書誌事項
Molecular biology and biotechnology
（Molecular and cell biochemistry）
Chapman & Hall, 1991
1st ed
- : pbk.
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This book is one of a series of brief fundamental texts for junior under- graduates and diploma students in biological science. The series, Molecular and Cell Biochemistry, covers the whole of modern biochemistry, integrating animal, plant and microbial topics. The intention is to give the series special appeal to the many students who read biochemistry for only part of their course and who are looking for an all-encompassing and stimulating approach. Although all books in the series bear a distinct family likeness, each stands on its own as an independent text. Many students, particularly those with less numerate backgrounds, find elements of their biochemistry courses daunting, and one of our principal concerns is to offer books which present the facts in a palatable style. Each chapter is prefaced by a list of learning objectives, with short summaries and revision aids at the ends of chapters. The text itself is informal, and the incorporation of marginal notes and information boxes to accompany the main text give a tutorial flavour, complementing and supporting the main narrative.
The marginal notes and boxes relate facts in the text to applicable examples in everyday life, in industry, in other life sciences and in medicine, and provide a variety of other educational devices to assist, support, and reinforce learning. References are annotated to guide students towards effective and relevant additional reading.
目次
1 DNA replication and repair.- 1.1 Introduction.- 1.2 Replication of DNA.- 1.3 Proofreading during DNA synthesis.- 1.4 Mutation and repair of DNA.- 1.5 Overview.- Answers to exercises.- Questions.- 2 Roles of RNA.- 2.1 Introduction.- 2.2 Ribosomal RNA.- 2.3 Transfer RNA.- 2.4 Small nuclear and cytoplasmic RNAs.- 2.5 Overview.- Answers to exercises.- Questions.- 3 Transcription and the genetic code.- 3.1 Introduction.- 3.2 Transcription: the basics.- 3.3 Transcription in prokaryotes.- 3.4 Transcription in eukaryotes.- 3.5 Reverse transcription.- 3.6 The genetic code.- 3.7 Overview.- Answers to exercises.- Questions.- 4 Protein biosynthesis.- 4.1 Introduction.- 4.2 Messenger RNA (mRNA).- 4.3 Transfer RNA (tRNA) and aminoacyl-tRNA synthetases.- 4.4 The ribosome cycle.- 4.5 Fidelity of translation.- 4.6 Post-translational modifications.- 4.7 Inhibitors of translation.- 4.8 Overview.- Answers to exercises.- Questions.- 5 Post-transcriptional and post-translational modifications.- 5.1 Introduction.- 5.2 mRNA processing.- 5.3 Transport of processed mRNA to the cytoplasm.- 5.4 Post-translational modifications of proteins.- 5.5 Overview.- Answers to exercises.- Questions.- 6 Induction and repression.- 6.1 Introduction.- 6.2 The lactose operon.- 6.3 Catabolite repression.- 6.4 The tryptophan operon.- 6.5 Attenuation.- 6.6 Positive and negative control.- 6.7 The arabinose operon.- 6.8 Overview.- Answers to exercises.- Questions.- 7 The eukaryotic chromosome.- 7.1 Introduction.- 7.2 Size of eukaryotic genomes: the C-value paradox.- 7.3 Specialized regions of chromosomes.- 7.4 Gene expression in eukaryotes.- 7.5 Levels of control of gene expression in eukaryotes.- 7.6 Control of transcription of eukaryotic genes.- 7.7 Mechanisms of transcription initiation.- 7.8 Overview.- Answers to exercises.- Questions.- 8 Mutation.- 8.1 Introduction.- 8.2 Mutation at the level of DNA.- 8.3 Mutant characters and mutant proteins.- 8.4 Spontaneous mutations.- 8.5 Induced mutations.- 8.6 Selection of mutants.- 8.7 Mechanisms to counter mutation.- 8.8 Uses of mutations.- 8.9 Overview.- Answers to exercises.- Questions.- 9 Recombinant DNA technology.- 9.1 Introduction.- 9.2 Transformation, plasmids and restriction endonucleases.- 9.3 Genetic engineering.- 9.4 Cloning of genes.- 9.5 DNA sequencing.- 9.6 Applications of genetic engineering.- 9.7 Recombinant DNA in forensic science.- 9.8 Prenatal diagnosis of human inherited genetic disorders.- 9.9 Overview.- Answers to exercises.- Questions.- 10 Cell culture and biotechnology.- 10.1 Introduction.- 10.2 The beginnings of animal and plant cell culture.- 10.3 Animal cell culture.- 10.4 Plant cell culture.- 10.5 The scale-up of animal and plant cell cultures.- 10.6 Animal cell products.- 10.7 Plant cell products.- 10.8 Overview.- Answers to exercises.- Questions.- Answers to questions.
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