Cytokines : a practical approach
著者
書誌事項
Cytokines : a practical approach
（The practical approach series）
IRL Press at Oxford University Press , Oxford University Press, c1991
- : hard
- : pbk.
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
As soluable mediators of immunity, inflammation and cell growth, cytokines are being studied in many areas of biological and medical research. They are also being used with increasing success for the treatment of malignant disease, chronic virus and parasitic infections, and some are rare congenital immune deficiencies. The recent indentification of naturally occuring cytokine inhibitors, binding either the cytokine of its receptor has opened up another realm of therapeutic potential. This book provides a catalogue of techniques that are applicable to cytokine research. Because of the multidisciplinary nature of cytokine reearch and the overlapping biologic properties of cytokines in vitro, each cytokine is not allocated an individual chapter. Instead, the book selects a number of biologic models and outlines the ways of assaying cytokine activity in these systems. In addition the book provides a spectrum of techniques for assaying cytokine production at the level of messenger RNA and protein, whether the sample be single cells, tissue sections, tissue cultures, sulture supernatants or clinical specimens.
Strategies for defining, purifying and cloning new cytokine genes are also included. In all, over 130 different techniques are in in this book, ranging from the basic to the highly sophisticated.
目次
- Cloning and expression of cytokine genes, R.Contreras et al
- southern blotting of cytokine genes, M.O.Diaz and T.W.McKeithan
- northern analysis and in situ hybridization of cytokine messenger RNAC, M.S.Naylor and R.Balkwill
- cytokine mapping - observation and quantification of cytokine mRNA in small numbers of cells using the polymerase chain reaction, A.Brenner et al
- receptor signaling in B lymphocytes, K.Rigley and R.Hicks
- two-dimensional gel electrophoresis and computer-based analysis of cytokine-induced proteins, L.B.Epstein et al
- receptor-binding studies, T.M.Mariano and S.Pestka
- antiviral activity of cytokines, J.A.Lewis
- human B-cell responses to cytokines, R.E.Callard
- T cells and cytokines, M.Londei et al
- the generation and quantitation of cell-mediated cytotoxicity, W.Loudon and E.Grimm
- assays for chemotaxis, J.Van Damme and R.Conings
- measurement of locomotion and the cytosolic calcium concentration in human polymorphonuclear leukocytes, J.Westwick
- assays for interleukin-5 (IL-5) and other regulators of eosinophil differentiation, M.Strath et al
- cytokine regulation of endothelial cells, E.Dejana et al
- assays for haematopoietic growth factors, N.G.Testa et al
- macrophage activation assays, M.Stein et al
- measurement of the proliferative effects of cytokines, E.Rozengurt
- measurement of cytokytic and cytostatic activity of cytokines, F.Burke and F.Balkwill
- development of antibodies to cytokines, S.Poole
- cytokine production by individual cells, C.E.Lewis
- RIA, IRMA and ELISA assays for cytokines, A.Meager
- quantitative biological assays for individual cytokines, M.Wadhwa et al.
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