Techniques for the rapid detection of plant pathogens and diagnosis of plant disease

著者
    • Duncan, J. M.
    • Torrance, L.
書誌事項

Techniques for the rapid detection of plant pathogens and diagnosis of plant disease

edited by J.M. Duncan & L. Torrance

Published for the British Society of Plant Pathology by Blackwell Scientific Publications, 1991

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内容説明・目次

内容説明

This book describes various rapid techniques, based primarily on immunology and nucleic acid probes, which could be used to detect the presence of pathogens, contaminants and toxins in plants and plant products, and to diagnose diseases caused by a variety of agents. Protocols of the various techniques, as used in the laboratory or in commercial concerns, are discussed and assessed.

目次

  • Part 1 Immunological techniques: serological methods to detect plant viruses
  • production and use of monoclonal antibodies
  • immunodiagnostic techniques for plant mycoplasma-like organisms
  • detection of plant invading fungi by monoclonal antibodies
  • assay methods for mycotoxins using monoclonal antibodies
  • techniques for detecting and identifying plant pathogenic bacteria. Part 2 Nucleic acid techniques: application of double stranded RNA analysis of plants to detect viruses, virus-like agents, virus satellites and sub-genomic viral RNAs
  • the detection of viroids and viruses by nucleic acid probes
  • identification of plant pathogenic bacteria using nucleic acid technology
  • use of RFLPs to identify races of fungal pathogens. Part 3 Commercial aspects and practical applications
  • screening for pathogens and contaminating micro-organisms in micropropagation
  • application of rapid techniques to seed health testing - prospects and potential
  • from the research bench to the market place
  • development of commercial diagnostic kits
  • commercialisation of serological tests for plant viruses.

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