Electron microscopy in biology

著者

• Harris, James E.

書誌事項

Electron microscopy in biology

edited by Robin Harris

（The practical approach series）

Oxford University Press, c1991

• pbk

注記

Includes bibliographical references and index

内容説明・目次

巻冊次

pbk ISBN 9780199632152

内容説明

Transmission electron microscopy is one of the most important and widely used forms of microscopy in biomedical research. Electron Microscopy in Molecular Biology : A Practical Approach was published in 1987, and contained techniques relating specifically to nucleic acids and proteins. The present volume provides detailed protocols for a wide range of procedures relating to the electron microscopy of tissues, cells, and their components with the emphasis on biomedical applications. The fundamental methods for specimen preparation, tissue fixation, embedding, and sectioning are covered in depth and are followed by the more specialist techniques of freeze-substitution, immunogold labelling, autoradiography, and enzyme histochemistry. Although the technical details in the book relate mainly to tissues and cells, many of the procedures can be adapted for isolated organelles and membrane fractions, viral particles, macromolecular assemblies, and large proteins. This is a broadly based compilation of the most important specimen preparation techniques and their applications. It will be of interest to researchers and advanced students working in almost every biomedical discipline, plus technicians in electron microscopy units.

目次

• David Hopwood & Gordon Milne: Fixation
• Michael Smith & Simon Croft: Embedding and thin section preparations
• Norbert Roos: Freeze-substitution and other low temperature embedding methods
• Alex D. Hyatt: Immunogold labelling techniques
• Gerard Morel: Electron microscopic autoradiographic techniques
• Corenelius J. F. Van Noorden & Caesar E. Hulstaert: Electron microscopical enzyme histochemistry
• Henry S. Slayter: High resolution shadowing
• Seizo Fujikawa: Freeze-fracture techniques
• Robin Harris & Robert Horne: Negative staining
• Murray Stewart: Transmission electron microscopy of vitrified biological macromolecular assemblies
• Alice Warley & Brij L. Gupta: Quantitative biological x-ray microanalysis
• Nigel James: Three dimensional electron microscopy of tissues and cells
• Appendix
• Index.

巻冊次

ISBN 9780199632190

内容説明

Transmission electron microscopy is one of the most important and popular forms of microscopy used in biomedical research. "Electron Microscopy - A Practical Approach" was published in 1987, and contained techniques relating specifically to nucleic acids and proteins. This volume provides protocols for a range of procedures relating to the electron microscopy of tissues, cells and their components with the emphasis on biomedical applications. The fundamental methods for specimen preparation, tissue fixation, embedding and sectioning are covered and are followed by the more specialist techniques of freeze-substitution, immunogold labelling, autoradiography and enzyme histochemistry. Although the technical details in the book relate mainly to tissues and cells, many of the procedures can be adapted for isolated organelles and membrane fractions, viral particles, macromolecular assemblies and large proteins. This is a broadly based compilation of the most important specimen preparation techniques and their applications. It is designed for researchers and advanced students working in almost every biomedical discipline, plus technicians in electron microscopy units.

目次

• Fixation, David Hopwood and Gordon Milne
• embedding and thin section preparations, Michael Smith and Simon Croft
• freeze-substitution and other low temperature embedding methods, Norbert Roos
• immunogold labelling techniques, Alex D.Hyatt
• electron microscopic autoradiographic techniques, Gerard Morel
• electron microscopical enzyme histochemistry, Corenelius J.F.van Noorden and Caesar E.Hulstaert
• high resolution shadowing, Henry S.Slayter
• freeze-fracture techniques, Seizo Fujikawa
• negative staining, Robin Harris and Robert Horne
• transmission electron microscopy of vitrified biological macromolecular assemblies, Murray Stewart
• quantitative biological x-ray microanalysis, Alice Warley and Brij L.Gupta
• three dimensional electron microscopy of tissues and cells, Nigel James.

「Nielsen BookData」 より

詳細情報

• NII書誌ID(NCID): BA13662952
• ISBN:
  • 0199632197
  • 0199632154
• LCCN: 91002476
• 出版国コード: uk
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: Oxford ; New York
• ページ数/冊数: xix, 308 p.
• 大きさ: 25 cm
• 分類:
  • DC20 : 578.45
  • NDC8 : 460.72
• 親書誌ID:
  • BA00227768