Site-directed mutagenesis and protein engineering : proceedings of the International Symposium on Site-Directed Mutagensis and Protein Engineering, Tromsø, 27-30 August 1990

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書誌事項

Site-directed mutagenesis and protein engineering : proceedings of the International Symposium on Site-Directed Mutagensis and Protein Engineering, Tromsø, 27-30 August 1990

editor, M. Rafaat El-Gewely

Elsevier Science Publishers, 1991

  • alk. paper

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注記

Includes bibliographical references and indexes

内容説明・目次

内容説明

This book primarily tackles the issues concerning the factors controlling the formation of the active form of proteins. Protein engineering is a multi-disciplinary field emerging as a result of interaction between fields of research such as: X-ray crystallography, biochemistry, molecular biology, and genetics. An overview of these interactions and their connection to protein engineering is presented in this volume, pointing the way to future research where protein engineers of the future will be able to design proteins of any needed function.

目次

Molecular structure and dynamics. Molecular structure and dynamics: Introduction (A. Hordvik). Molecular structure of neurotransmitter receptors and ligands (S.G. Dahl). The active site and catalytic mechanism in phospholipase C from Baccillus cereus (E. Hough). PDBase: Poor man's structural protein database tool (S.B. Petersen). Electrostatics of proteins (P. Martel and S.B. Petersen). Applications of mass spectrometry in biotechnology (J.S. Svendsen). Neural networks applied to the study of protein sequences and protein structures (H. Fredholm, H. Bohr, J. Bohr, S. Brunak, R.M.J. Cotterill, B. Lautrup and S.B. Petersen). Protein engineering. Protein engineering (D.L. Oxender and T.J. Graddis). High-level expression of bovine pancreatic RNase A (K. Trautwein and S.A. Benner). Molecular analysis of leucine-binding protein specificity (D.L. Oxender and M.D. Adams). The mechanism of pancreatic phospholipase A2 studied with recombinant DNA techniques (H.M. Verheij). Processing and stability studies of recombinant human parathyroid hormone by in vitro mutagenesis (K.M. Gautvik, B.N. Kareem, S. Reppe, E. Rokkones, O.K. Olstad, O.S. Gabrielsen, A. Hogset, O.R. Blingsmo, V.T. Gautvik, P. Alestrom and T.B. Oyen). Site-directed mutagenesis of glutaredoxin from bacteriophage T4 (T. Joelson, M. Nikkola, M. Ingelman, O. Bjornberg, B.-M. Sjoberg and H. Eklund). Mutational analysis of a transcriptional regulatory protein of bacteriophage P2 (K. Gebhart and B.H. Lindqvist). In vitro mutagenesis of rat catechol-o-methyltransferase (K. Lundstrom, H. Ahti and I. Ulmanen). Application of the polymerase chain reaction to DNA engineering (K.Hagen-Mann). DNA-protein interactions. Correlation between the homologies of TthHB8 and Taq1 isoschizomers and insertion mutants in the Taq1 endonuclease gene (J. Zebala, A. Mayer and F. Barany). A genetic approach to study the structure-function relationship of tryptophan repressor (M.R. El-Gewely). Rules for protein/DNA-recognition (N. Lehming, J. Sartorius, B. Kisters-Woike, B. van Wilcken-Bergmann and B. Muller-Hill). Site-directed mutagenesis. Oligonucleotide and multi site-directed mutagenesis (M.R. El-Gewely). Phosphorothioate based mutagesesis of single and double-stranded DNA vectors (D.B. Olsen and J.R. Sayers). Mapping catalytically important regions in beta-lactamase using two codon insertion mutagenesis (J. Zabala and F. Barany). Polymerase chain reaction: A strategy for successful amplification (K. Hagen-Mann). Screening of cDNA-libraries and gene reconstruction by PCR (B.Y. Nordvag, I. Nilsen, G. Husby and M.R. El-Gewely). Chemical modification may be used to stabilize en enzyme (C. O'Fagain and R. O'Kennedy). Index of authors. Subject index.

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