Functional organization of the nucleus : a laboratory guide
著者
書誌事項
Functional organization of the nucleus : a laboratory guide
（Methods in cell biology / edited by David M. Prescott, v. 35）
Academic Press, c1991
- : hbk
- : pbk
大学図書館所蔵 全24件
注記
Includes bibliographical references and index
内容説明・目次
- 巻冊次
-
: pbk ISBN 9780123219206
内容説明
The complex problem of the relationship between nuclear structure and function demands a multidisciplinary, multifaceted approach. This laboratory guide is designed for researchers, from graduate students to professors, who need detailed protocols and general discussions on a broad range of techniques. The volume presents a selection of different methodological approaches for the analysis of nuclear structure and function - from cytological to molecular to genetic. These include visualization of nucleic acid sequences using hybridization probes, visualization of proteins using immunological probes, isolation of chromatin fractions, mapping in vitro protein-DNA interactions, reconstitution of functional templates and nuclear substructures, and genetic approaches to identifying and characterizing chromosomal components.
目次
- DNA sequence localization in metaphase and interphase cells by fluorescence in situ hybridization, B. J. Trask
- localization of mRNAs by in situ hybridization, L. M. Angerer and R. C. Angerer
- fluorescent detection of nuclear RNA and DNA - implications for genome organization, C. V. Johnson et al
- visualization of DNA sequences in meiotic chromosomes, P. B. Moens and R. E. Pearlman
- nucleic acid sequence localization by EM in situ hybridization , S. Narayanswami et al
- the use of autoantibodies in the study of nuclear and chromosomal organization, W. C. Earnshaw and J. B. Rattner
- meiotic chromosome preparation and protein labeling, C. Heyting and A. J. J. Dietrich
- distribution of chromosomal proteins in polytene chromosomes of drosphila, R. F. Clark et al
- the use of nonoclonal antibody libraries, H. Saumweber
- optical sectioning and three-dimensional reconstruction of diploid and polytene nuclei, M. C. Rykowski
- yeast minichromosomes, S. Y. Roth and R. T. Simpson
- nucleosomes of transcriptionally active chromatic - isolation of template-active nucleosomes by affinity chromatography, V. G. Alfrey and T. A. Chen
- the nucleoprotein hybridization method for isolating active and inactive genes as chromatin, C. Vincenz et al
- protein-DNA crosslinking as a means to determine the distribution of proteins on DNA in vivo, D. S. Gilmour et al
- protein-DNA interactions in vivo - examining genes in saccharaomyces cerevisiae and drosophila melanogaster by chromatin footprinting, M. W. Hull et al
- control of Class II gene transcription during in vitro nucleosome assembly, J. L. Workman et al
- systems for the study of nuclear assembly, DNA replication, and nuclear breakdown in xenopus laevis egg extracts, C. Smythe and J. W. Newport
- in vitro nuclear protein import using permeabilized mammalian cells, S. A. Adam et al
- mutations that affect chromosomal proteins in yeast, M. M. Smith
- mutations that affect nuclear organization in yeast, A. O. Sperry et al
- mutations that affect cell division in drosophila, M. Gatti and M. L. Goldberg
- position effect variegation - an assay for mutations in chromosomal proteins and chromatin assembly in drosophila, T. Grigliatti.
- 巻冊次
-
: hbk ISBN 9780125641357
内容説明
A text intended for researchers and students in cell biology, genetics and molecular biology, developmental biology, biochemistry and biophysics. The complex problem of the relationship between nuclear structure and function demands a multidisciplinary, multifaceted approach. This laboratory guide is designed for researchers, from graduate students to professors, who need detailed protocols and general discussions on a broad range of techniques. The volume presents a selection of different methodological approaches for the analysis of nuclear structure and function - from cytological to molecular to genetic. These include visualization of nucleic acid sequences using hybridization probes, visualization of proteins using immunological probes, isolation of chromatin fractions, mapping "in vitro" protein-DNA interactions, reconstitution of functional templates and nuclear substructures, and genetic approaches to identifying and characterizing chromosomal components.
目次
- DNA sequence localization in metaphase and interphase cells by fluorescence "in situ" hybridization, B.J. Trask
- localization of mRNAs by "in situ" hybridization, L.M. Angerer and R.C. Angerer
- fluorescent detection of nuclear RNA and DNA - implications for genome organization, C.V. Johnson et al
- visualization of DNA sequences in meiotic chromosomes, P.B. Moens and R.E. Pearlman
- nucleic acid sequence localization by EM "in situ" hybridization, S. Narayanswami et al
- the use of autoantibodies in the study of nuclear and chromosomal organization, W. C. Earnshaw and J.B. Rattner
- meiotic chromosome preparation and protein labeling, C. Heyting and A.J.J. Dietrich
- distribution of chromosomal proteins in polytene chromosomes of "drosophila", R.F. Clark et al
- the use of monoclonal antibody libraries, H. Saumweber
- optical sectioning and three-dimensional reconstruction of diploid and polytene nuclei, M.C. Rykowski
- yeast minichromosomes, S.Y. Roth and R.T. Simpson
- nucleosomes of transcriptionally active chromatin - isolation of template-active nucleosomes by affinity chromatography, V.G. Allfrey and T.A. Chen
- the nucleoprotein hybridization method for isolating active and inactive genes as chromatin, C. Vincenz et al
- protein-DNA crosslinking as a means to determine the distribution of proteins on DNA "in vivo", D.S. Gilmour et al
- protein-DNA interactions "in vivo" - examining genes in "saccharomyces cerevisiae" and "drosophila melanogaster" by chromatin footprinting, M.W. Hull et al
- control of class II gene transcription during "in vitro" nucleosome assembly, J.L. Workman et al
- systems for the study of nuclear assembly, DNA replication and nuclear breakdown in "xenopus laevis" egg extracts, C. Smythe and J.W. Newport
- "in vitro" nuclear protein import using permeabilized mammalian cells, S.A. Adam et al
- mutations that affect chromosomal proteins in yeast, M.M. Smith
- mutations that affect nuclear organization in yeast, A.O. Sperry et al
- mutations that affect cell division in "drosophila", M. Gatti and M.L. Goldberg
- position effect variegation - an assay for mutations in chromosomal proteins and chromatin assembly in "drosophila", T. Grigliatti.
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