CiNii 図書 - Molecular plant pathology : a practical approach

Advances in the understanding of cellular processes at the molecular level have transformed the biological sciences. Plant pathology is a field which has benefitted dramatically from the application of molecular techniques and has become one of the most exciting areas of biology today. "Molecular Plant Pathology: a Practical Approach Volume I" and its companion Volume II provide a comprehensive guide to the latest molecular techniques as well as the established approaches to the subject. Detailed protocols are included which address a wide range of investigations from plant pathogen isolation and culture, through physiology and biochemistry, to techniques for localizing genes and their products within the cells of the infected plants. Topics covered in volume I include, introduction of cloned DNA into plant pathogenic bacteria, gene tranformation in plant pathogenic fungi, nucleic acid isolation and hybridization techniques, and in situ hybridzation in plants.

Plant viruses, R.Hull
geminiviruses, P. Mullineaux
RNA-based viruses, P. Kroner and P. Ahlquist
bacteria, M.J. Daniels
introduction of cloned DNA into plant pathogenic bacteria, N.T. Keen, et al
RFLP analyses and gene tagging for bacterial identification and taxonomy, D.W. Gabriel and R. de Feyter
Fungi, J.M. Clarkson
gene transformation in plant pathogenic fungi, J. Hargreaves and G. Turner
nematodes, H.J. Atkinson
nucleic acid isolation and hybridization techniques, S.J. Gurr and M.J. McPherson
the polymerase chain reaction, M.J. McPherson and S.J. Gurr
analysis of defence gene transcriptional regulation, M.J. Harrison, et al
in-situ hyridization in plant, D. Jackson
gene expression using baculoviruses, I.R. Cameron and D.H.L. Bishop.

巻冊次: v. 2 : pbk ISBN 9780199633517

内容説明

Molecular plant pathology has directly benefited from advances in modern molecular techniques. These techniques have been applied both to pathogens and plants, enhancing our understanding of the organisms themselves and of the complex interactions which determine compatability between them and their host plants. This volume and its companion together provide the first comprehensive guide to the latest molecular techniques as well as the established approaches to the subject. Researchers in plant science, molecular biology, and related areas will find the two volumes of Molecular Plant Pathology to be an invaluable experimental resource for this exciting and fast-moving field, providing a wealth of easy to follow protocols supported by expert advice and guidance.

SECTION I: DEFENCE RESPONSE (GENE PRODUCTS)
1. Callose and callose synthase
2. Lignin and associated phenolic acids in cell walls
3. Preparation and analysis of intercellular fluid
4. Biochemical analysis of chitinases and -1,3- glucanases
5. Localization of proteins and carbohydrates using immunogold labelling in light and electron microscopy
6. Isoflavonoid phytoalexins and their biosynthetic enzymes
7. Analysis of terpenoid phytoalexins and their biosynthetic enzymes
SECTION II: ELICITORS
8. Preparation and characterization of oligosaccharide elicitors of phytoalexin accumulation
9. Xyloglucan oligosaccharides
SECTION III: SIGNAL TRANSDUCTION PATHWAYS
10. Analysis of calcium involvement in host-pathogen interactions
11. Analysis of components of the plant phosphoinositide system
12. Metabolic changes following infection of leaves by fungi and viruses
SECTION IV: DISEASE RESISTANCE GENES
13. Strategies for cloning disease resistance genes

巻冊次: v. 2 ISBN 9780199633524

内容説明

Molecular plant pathology has directly benefited from advances in modern molecular techniques. These techniques have been applied both to pathogens and plants, enhancing our understanding of the organisms themselves and of the complex interactions which determine compatability between them and their host plants. This volume and its companion together provide a comprehensive guide to the latest molecular techniques as well as the established approaches to the subject. Topics covered in in this second volume include: analysis of defence-related compounds; strategies for cloning disease resistance genes; characterization of elicitors; and signal transduction pathways. Researchers in plant science, molecular biology and related areas should find the two volumes of "Molecular Plant Pathology: A Practical Approach" to be an invaluable experimental resource for this exciting and fast-moving field, providing a wealth of easy-to-follow protocols supported by expert advice and guidance.

Part 1 Defence response (gene products): callose and callose synthase, H. Kauss
lignin and associated phenolic acids in cell walls, J. Friend
preparation and analysis of intercellular fluid, K.E. Hammond-Kosack
biochemical analysis of chitinases and beta-1,3- glucanases, T. Boller
localization of proteins and carbohydrates using immunogold labelling in light and electron microscopy, K.A. Van den Bosch
isoflavonoid phytoalexins and their biosynthetic enzymes, R. Edwards and H. Kessmann
analysis of terpernoid phytoalexins and their biosynthetic enzymes, D.R. Threlfall and I.M. Whitehead. Part 2 Elicitors: preparation and characterization of oligosaccharide elicitors of phytoalexin accumulation, M.G. Hahn et al
xyloglucan oligosaccharides, S.C. Fry. Part 3 Signal transduction pathways: analysis of calcium involvement in host-pathogen interactions, B.K. Drobak et al
analysis of components of the plant phosphoinositide system, B.K. Drobak and K. Roberts
metabolic changes following infection of leaves by fungi and viruses, R.C. Leegood and J.D. Scholes. Part 4 Disease resistance genes: strategies for cloning disease resistance genes, R.W. Michelmore et al.

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Molecular plant pathology : a practical approach

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