Electron microscopic immunocytochemistry : principles and practice

書誌事項

Electron microscopic immunocytochemistry : principles and practice

edited by Julia M. Polak and John V. Priestley

(Oxford science publications)

Oxford University Press, 1992

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

Electron microscopic immunocytochemistry is a powerful technique, combining the resolution of the electron microscope with the specificity and sensitivity of immunocytochemistry. Recent developments have simplified the techniques and broadened its range of applications, yet it is not used as widely as it could be, because it still has a reputation as an expensive and intricate procedure, beyond the grasp of most laboratories. This book aims to make the technique more accessible; it provides a thorough introduction to the principles of EM immunocytochemistry, detailed protocols, and examples of ways in which the technique can be applied to a variety of problems, in biomedical research and in diagnostic pathology. The emphasis throughout is on giving the practical help and guidance needed by scientists and clinicians who may be using the technique for the first time. The first part of the book provides an overview of EM immunocytochemical procedures and of applications in histopathology. Then three major chapters cover post-embedding, pre-embedding, and ultra-thin cryosection techniques. The final section illustrates applications of EM immunocytochemistry to the study of endocrine tumours, renal disease, neuropathology, dermatology, and microbiology.

目次

  • Introduction and overview of electron microscopic immunocytochemical procedures, J.V. Priestley
  • electron microscopic immunocytochemistry in histopathology, C.H.S. Cameron and P.G. Toner
  • post-embedding electron microscopic immunocytochemistry, A. Merighi
  • pre-embedding electron microscopic immunocytochemistry, J.V. Priestley et al
  • immunocytochemistry of cryosections, P. Monaghan and A. Atherton
  • electron microscopic immunocytochemistry of endoctrine tumours, A.E. Bishop and J.M. Polak
  • electron microscopic immunocytochemistry in renal disease, M.D. Al-Naweb and D.R. Davies
  • electron microscopic immunocytochemistry in neuropathology, J.W. Ironside
  • electron microscopic immunocytochemistry in dermatology, R.A.J. Eady
  • electron microscopic immunocytochemistry in microbiology, J.E. Beesley.

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