Crystallization of nucleic acids and proteins : a practical approach
著者
書誌事項
Crystallization of nucleic acids and proteins : a practical approach
（The practical approach series）
IRL Press at Oxford University Press, c1992
- : pbk
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注記
Includes bibliographical references and index
内容説明・目次
- 巻冊次
-
ISBN 9780199632459
内容説明
In the last decade crystallography has emerged as a key tool for understanding the structures of biological macromolecules, yet the crystallization techniques themselves have been perceived as difficult to perform. This book provides a detailed guide to producing crystals of proteins and nucleic acids for diffraction studies. Standard crystallization methods are given, along with procedures for the preparation and handling of macromolecules. Other topics covered include seeding procedures, crystallization in gels, under microgravity and supergravity, crystallization of nucleic acids, nucleic acid-protein complexes and membrane proteins, an introduction to X-ray crystallography and automated processes. Detailed laboratory protocols and the underlying theoretical background are brought together, providing researchers with the conceptual and methodological tools necessary to exploit these techniques.
目次
- An introduction to the crystallogenesis of biological macromolecules, R. Giege and A. Ducuix
- preparation and handling of biological macromolecules for crystallization, B. Lorber and R. Giege
- design of crystallization experiments and protocols, C.W. Carter
- methods of crystallization, A. Ducruix and R. Giege
- seeding techniques, E. Stura and I.A. Wilson
- crystallization in gels and related methods, M.C. Robert
- crystallization of nucleic acids and co-crystallization of proteins and nucleic acids, A.C. Dock-Bregeon and D. Moras
- crystallization of membrane proteins, F. Reiss-Husson
- phase diagrams, M. Ries-Kaut and A. Ducruix
- the physical chemistry of potein crystallization, N. Mikol.
- 巻冊次
-
: pbk ISBN 9780199632466
内容説明
In the last decade crystallography has emerged as a key tool for understanding the structures of biological macromolecules, yet the crystallization techniques themselves have been perceived as difficult to perform. This book provides the first detailed and rational guide to producing crystals of proteins and nucleic acids for diffraction studies. Standard crystallization methods are given, along with procedures for the preparation and handling of macromolecules.
Detailed laboratory protocols and the underlying theoretical background are brought together, providing researchers with the conceptual and methodological tools necessary to exploit these powerful techniques.
目次
- R. Giege & A. Ducruix: An introduction to the crystallogenesis of biological macromolecules
- B. Lorber & R. Giege: Preparation and handling of biological macromolecules for crystallization
- C.W. Carter: Design of crystallization experiments and protocols
- A. Ducruix & R. Giege: Methods of crystallization
- E. Stura & I.A. Wilson: Seeding techniques
- M.C. Robert, K. Provost, & F. Lefaucheux: Crystallization in gels and related methods
- A.C. Dock-Bregeon & D. Moras: Crystallization of nucleic acids and co-crystallization of proteins and nucleic acids
- F. Reiss-Husson: Crystallization of membrane proteins
- M. Ries-Kaut & A. Ducruix: Phase diagrams
- V. Mikol & R. Giege: The physical chemistry of protein crystallization
- E.A. Stura & P. Chen: Soaking of crystals
- L. Sawyer & M.A. Turner: X-ray analysis
- K.B. Ward, M.A. Perozzo & W.M. Zuk: Automating crystallization experiments
- S. Doublie & C.W. Carter: Preparation of selenomethionyl protein crystals
- Appendix
- Index.
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