書誌事項

Enzyme assays : a practical approach

edited by Robert Eisenthal and Michael J. Danson

(The practical approach series)

IRL Press at Oxford University Press, c1992

  • pbk.

大学図書館所蔵 件 / 20

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注記

Includes bibliographical references and indexes

内容説明・目次

巻冊次

ISBN 9780199631421

内容説明

Virtually all chemical reactions in living systems are catalysed by enzymes, and consequently, enzyme assays are probably one of the most frequently performed procedures in biochemistry. This book provides a wealth of protocols for use in all areas of the life science.

目次

  • K.F. Tipton: Principles of enzyme assay and kinetic studies
  • Robert John: Photometric assays
  • K.G. Oldham: Radiometric assays
  • S. Syed: High performance liquid chromatographic assays
  • P.D.J. Weitzman & P.J. Watkins: Electrochemical assays: polarography
  • J.B. Clark: Electrochemical assays: the oxygen electrode
  • K. Brocklehurst: Electrochemical assays: the pH-stat
  • O. Gabriel, & D.M. Gersten: Detection of enzymatic activity after polyacryladmide gel electrophoresis and agarose gel isoelectric focusing
  • N.C. Price: Techniques for enzyme extraction
  • P.J.F. Henderson: Statistical analysis of enzyme kinetic data
  • L. Stevens: Buffers and the determination of protein concentrations.
巻冊次

pbk. ISBN 9780199631438

内容説明

This book is intended for undergraduates and postgraduates in the biological and medical sciences. All researchers involved in the assay and characterization of enzymes.

目次

1: K. F. Tipton: Principles of Enzyme Assay and Kinetic Studies. 2: Robert John: Photometric Assays. 3: K. G. Oldham: Radiometric Assays. 4: S. Syed: High Performance Liquid Chromatographic Assays. 5: P. D. J. Weitzman and P. J. Watkins: Electrochemical Assays: Polorography. 6: J. B. Clark: Electrochemical Assays: The Oxygen Electrode. 7: K. Brocklehurst: Electrochemical Assays: The pH-Stat. 8: O. Gabriel and D. M. Gersten: Detection of Enzymatic Activity After Polyacryladmide Gel Electrophoresis and Agarose Gel Isoelectric Focusing. 9: N. C. Price: Techniques for Enzyme Extraction. 10: P. J. F. Henderson: Statistical Analysis of Enzyme Kinetic Data. 11: L. Stevens: Buffers and the Determination of Protein Concentrations. 12: References

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