Baculovirus expression vectors : a laboratory manual
著者
書誌事項
Baculovirus expression vectors : a laboratory manual
W.H. Freeman, c1992
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注記
Includes bibliographical references (p. 307-339) and index
内容説明・目次
内容説明
Baculoviruses have-proven-to be the most powerful and versatile eukaryotic expression vectors available. This laboratory manual is designed to help both beginning and experienced researchers construct and use baculovirus vector systems. "Baculovirus Expression Vectors" aims to simplify selection of the most appropriate vector design for a given problem. It describes each step of the implementation process from vector construction to large-scale protein production. The manual provides: an understanding of how the vectors work a biological overview of cells, viruses, plasmids, and promoters; guidelines for choosing optimum vectors protocols for growing insect cells and recombinant viruses; methods of analysing protein products and scaling up protein production; and techniques for producing proteins in insect larvae. The manual also includes easy-to-use maps charting available expression vectors.
目次
- An overview of baculoviruses
- choosing a transfer plasmid and parent virus
- methods for vector construction and gene expressions
- methods for scale-up of protein production and use of insect larvae. Appendices: nucleotide sequence of AcMNPV polyhedrin region
- nucleotide sequence of AcMNPV p10 Region
- list of genes expressed in the baculovirus expression system
- suppliers
- stock solutions
- Excel spreadsheet for TCID calculation.
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