Gene expression technology
Author(s)
Bibliographic Information
Gene expression technology
（Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan, v. 185）
Academic Press, c1991
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Description and Table of Contents
Description
The aim of this volume is to give recombinant DNA researchers the most up-to-date knowledge about heterologous gene expression in four commonly employed organisms: escherichia coli, bacillus subtilis, yeast and mammalian cells. The methods presented should enable the reader to design effective strategies for the expression of cloned genes and cDNAs, and prove useful in solving the majority of expression problems that are likely to be encountered.
Table of Contents
- Expression in escherichia coli
- promoters
- translation
- proteases
- gene fusions
- secretion
- protein refolding
- expression in bacillus subtilis
- intracellular production
- inducible expression of regulatory proteins
- expression in yeast
- plasmid vectors
- inducible expression cassettes
- constitutive expression cassettes
- translation
- proteases
- protein secretion and modification
- expression in mammalian cells
- transfection methods
- markers for selection and amplification
- growth of cell lines
- posttranslation processing, modification, and secretion
- mutagenesis.
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