Animal cell culture : a practical approach
Author(s)
Bibliographic Information
Animal cell culture : a practical approach
（The practical approach series）
IRL Press at Oxford University Press, c1992
2nd ed
- : hbk
- : pbk
Available at / 19 libraries
-
Obihiro University of Agriculture and Veterinary Medicine Library図
: hbk039726,
481.1/F46020397267 -
University Library for Agricultural and Life Sciences, The University of Tokyo図
: hbk613.63:F46:2d-ed5019264364
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Note
Includes bibliographical references and index
Description and Table of Contents
Description
This new edition takes account of the progress that has been made in the field since the publication of the first edition in 1986. Although the basic principles remain the same, significant advances have been made in areas such as serum-free media, scale-up, and flow cytometry. As these techniques have developed as tools for the cell biologist, their availability to the non-specialist has also increased dramatically. Use of the tetrazolium salt MTT as a colorimetric indicator of viability has made a considerable impact on cytotoxicity assay, and DNA fingerprinting has revolutionized the identification of individual cell strains. These, and other developments in the techniques described have made this new edition essential. The emphasis remains on presenting techniques in a readily accessible form, with detailed protocols given throughout. This volume should be of use to researchers involved in both biological research and the commercial exploitation of animal cell culture.
Table of Contents
- R.I. Freshney: Introduction to basic principles
- H.R. Maurer: Towards serum-free, chemically defined media for mammalian cell culture
- B. Griffiths: Scaling-up of animal cell cultures
- R.J. Hay: Cell line preservation and characterization
- D. Conkie: Separation of viable cells by centrifugal elutriation
- J.V. Watson & E. Erba: Flow cytometry
- I. Lasnitzki: Organ culture
- A.P. Wilson: Cytotoxicity and viability assays
- S.M. Lang, A.H. Wyllie, & D. Conkie: In situ hybridization
- Index.
by "Nielsen BookData"
