Pulsed-field gel electrophoresis : protocols, methods, and theories
著者
書誌事項
Pulsed-field gel electrophoresis : protocols, methods, and theories
（Methods in molecular biology / John M. Walker, series editor, 12）
Humana Press, c1992
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Burmeister and Ulanovsky's new volume on "Pulsed-Field Gel Electrophoresis" presents step-by-step protocols for all users of this powerful methodology, whether novice or expert. The book features a wide range of PFGE techniques, auxiliary methods, and a diverse array of powerful applications. Each protocol is given thorough treatment by authors who have had extensive hands-on-experience with it. Indeed, many of the contributors have long provided assistance and troubleshooting advice to new users of PFGE during the early stages of its growth. Here they incorporate the benefits of their experience in the extensive Notes sections, which describe common problems and pitfalls and offer sensible solutions for dealing with them. Topics and techniques treated include: FIGE, CHEF, OFAGE, TAFE, FORE, ODPFGE; preparation of DNA suitable for PFGE from: bacteria, protozoa, plants, mammals, and yeasts; preparation and analysis of yeast artificial chromosomes (YACS); mapping strategies; biological applications; and, theories and observations.
In keeping with the well-known practicality of Humana's "Methods in Molecular Biology" series, "Pulsed-Field Gel Electrophoresis" brims with protocols designed to work even the first time they are used.
目次
Electrophoretic Techniques. Field-Inversion Gel Electrophoresis. Resolving Multi-megabase DNA Molecules Using Contour-Clamped Homogeneous Electric Fields (CHEF). Separation and Size-Determination of DNA over a 10-200 kbp Range. PFGE Using Double-Inhomogeneous Fields or Orthogonal Field-Alternating Gel Electrophoresis (OFAGE). Transverse Alternating-Field Electrophoresis. Rotating Field Gel Electrophoresis (ROFE). Preparation, Manipulation, and Pulse Strategy for One-Dimensional Pulsed-Field Gel Electrophoresis (ODPFGE). Auxiliary Methods. Preparation, Restriction, and Hybridization Analysis of Mammalian Genomic DNA for Pulsed-Field Electrophoresis. Encapsulation of Cells in Agarose Beads. Preparation and Separation of Intact Chromosomes of Vertebrates by One-Dimensional Pulsed-Field Gel Electrophoresis (ODPFGE). Isolation of Plant DNA for Pulsed-Field Gel Electrophoresis. Methyltransferases as Tools to Alter the Specificity of Restriction Endonucleases. Desktop Digital Imaging: Application to Detection of Length Heterogeneity After Hyperresonant Pulsed-Field Gel Electrophoresis of Mature Bacteriophage P22 DNA. Biological Applications. Bacterial Genome Mapping by Two-Dimensional Pulsed Field Gel Electrophoresis (2D-PFGE). Protozoan Genomes: Karyotype Analysis, Chromosome Structure, and Chromosome Specific Libraries. Construction of Yeast Artificial Chromosome Libraries by Pulsed-Field Gel Strategies for Mapping Large Regions of Mammalian Genomes. Two-Dimensional DNA Electrophoresis (2D-DE) for Mammalian DNA. Pulsed-Field and Two-Dimensional Gel Electrophoresis of Long Arrays of Tandemly from Large PFGE Fragments. In-Gel Detection of DNA: Application to Study of Viral DNA Metabolism by Use of Pulsed-Field Agarose Gel Electrophoresis. Theories and Observations. Theories of Pulsed-Field Gel Electrophoresis. The Dynamics of DNA Gel Electrophoresis. Orientation of the Agarose Matrix by Pulsed Electric Fields. Reptation Theory of Pulsed Electrophoresis and Trapping Electrophoresis. Elastic Bag Model of One-Dimensional Pulsed-Field Gel Electrophoresis (ODPFGE). Index.
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