Luminescent spectroscopy of proteins
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書誌事項
Luminescent spectroscopy of proteins
CRC Press, c1993
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Luminescent Spectroscopy of Proteins is devoted to the method of intrinsic protein luminescence, one of the most popular experimental methods in modern biophysics and biochemistry. The book discusses general physical principles of the luminescence method; spectral properties of the main protein chromophores; and protein luminescence and its use for studies on structural, physico-chemical, and functional properties of proteins. Principles of luminescent spectroscopy are illustrated by real-life applications and problems. Luminescent Spectroscopy of Proteins will be an excellent reference for biophysicists, biochemists, analytical chemists, and other scientists interested in this topic.
目次
INTRODUCTION. ENERGY LEVELS IN MOLECULES AND TRANSITIONS BETWEEN THEM. ENERGY LEVELS. INTERNAL CONVERSION AND VIBRATIONAL RELAXATION. FLUORESCENCE. INTERSYSTEM CROSSING. PHOSPHORESCENCE. FRANK-CONDON PRINCIPLE. LUMINESCENCE LIFETIME. FLUORESCENCE QUANTUM YIELD. STERN-VOLMER EQUATION. EXCITATION ENERGY TRANSFER BETWEEN SINGLET STATES. EFFECTS OF INTERMOLECULAR INTERACTIONS ON ELECTRON-VIBRATIONAL TRANSITIONS IN MOLECULES. SCHEME OF STEADY-STATE SPECTROFLUORIMETER. SPECTROSCOPIC PROPERTIES OF ISOLATED PROTEIN CHROMOPHORES. TRYPTOPHAN. Absorption Spectrum. Emission Spectra. Emission Lifetime. Analytical Description of the Fluorescence Spectrum Shape. Fluorescence Quantum Yield. Acid-Base Properties in the Ground and Excited State. Fluorescence Quenching by Protein Groups. Temperature Dependence of Bimolecular Quenching of Indole Fluorescence. TYROSINE. Absorption Spectrum. Emission Spectra. Emission Lifetime. Fluorescence Quenching by Protein Groups. PHENYLALANINE. Absorption Spectrum. Emission Spectrum. Fluorescence Quantum Yield. PROTEIN LUMINESCENCE. POSITION AND SHAPE OF PROTEIN FLUORESCENCE SPECTRA. Tryptophan Fluorescence. Tyrosine Fluorescence. Phenylalanine Fluorescence. FLUORESCENCE QUANTUM YIELDS OF PROTEINS. Tryptophan Fluorescence. Tyrosine Fluorescence. Phenylalanine Fluorescence. FLUORESCENCE DECAY OF PROTEINS. Tryptophan Fluorescence. Tyrosine Fluorescence. Phenylalanine Fluorescence. GENERAL STRATEGY OF LUMINESCENT STUDY OF PROTEINS. FLUORESCENCE PARAMETERS AS A MEASURE OF THE FRACTION OF CONVERSION FROM ONE STATE TO ANOTHER IN PROTEINS. SEARCH FOR INTERMEDIATE STATES. STUDIES OF TEMPERATURE DEPENDENCIES OF PROTEIN FLUORESCENCE PARAMETERS. STUDIES OF PH-DEPENDENCIES OF PROTEIN FLUORESCENCE. STUDIES OF EFFECTS OF IONIC STRENGTH ON PROTEIN FLUORESCENCE. STUDIES OF EFFECTS OF DENATURANTS ON PROTEIN FLUORESCENCE. STUDIES OF INTERACTIONS OF PROTEINS WITH LOW MOLECULAR MASS COMPOUNDS BY FLUORESCENCE METHOD. METHOD OF SELECTIVE FLUORESCENCE QUENCHING. LOW TEMPERATURE LUMINESCENCE OF PROTEINS. LITERATURE. Captions to Figures. Tables. Subject Index.
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